SAB4200540
Anti-HAP1 antibody produced in rabbit
IgG fraction of antiserum
Synonym(s):
Anti-HAP2, Anti-HIP5, Anti-hHLP1, Anti-huntingtin-associated protein 1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~70 kDa
species reactivity
rat, human
technique(s)
indirect immunofluorescence: 1:100-1:200 using PC-12 cells differentiated with rat NGF.
western blot: 1:1,000-1:2,000 using lysates of A431 cells
UniProt accession no.
shipped in
dry ice
Storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HAP1(9001)
rat ... 29430(Hap1)
General description
Htt-associated protein-1 (HAP1) is present abundantly in the brain. It exists in two isoforms and is located on the human chromosome at 17q21.2.
The gene HAP1 (huntingtin-associated protein 1) encodes a nuclear enzyme that is mainly expressed in neurons. It is the human homolog of exonuclease III protein from E. coli. The gene is mapped to human chromosome 17q21.2-q21.3.
Specificity
Anti-HAP1 specifically recognizes human and rat HAP1.
Immunogen
synthetic peptide corresponding to an internal sequence of human HAP1, conjugated to KLH. Whole antiserum is purified using protein A immobilized on agarose to provide the IgG fraction of antiserum.
Application
Anti-HAP1 antibody produced in rabbit may be used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Htt-associated protein-1 (HAP1) binds to the Htt protein due to PolyQ expansion. It plays a role in intracellular trafficking in neurons. HAP1 also functions as an adaptor protein linking vesicles with intracellular transporters. It interacts with dynactin p150Glued and kinesin light chain (KLC). HAP1 takes part in endosomal trafficking and stabilization of internalized epidermal growth factor receptor (EGFR) and γ-aminobutyric acid A receptor (GABAAR). Modulation of HAP1 trafficking is crucial for the stability of tropomyosin-related kinase A (TrkA) and neurite outgrowth. HAP1 is also involved in the interaction with pro-brain-derived neurotrophic factor (pro-BDNF) and mediates its transport and release. HAP-1 is associated with pathogenesis of Huntington′s disease.
The gene HAP1 (huntingtin-associated protein 1) is a bifunctional enzyme that functions as a DNA repair enzyme as well as in redox activation of transcription factors. It binds to the Huntington′s disease (HD) protein huntingtin in a polyglutamine length-dependent manner. It associates with huntingtin via the cytoskeletal proteins dynactin and pericentriolar autoantigen protein 1 forming coiled-coils. It plays a role in vesicle trafficking by facilitating interactions among cytoskeletal, vesicular and motor proteins. It is an apurinic/apyrimidinic (AP) site-specific DNA repair endonuclease that removes the AP sites produced naturally or by cytotoxic drugs and radiation. It also has RNAse H activity and controls the redox state of certain proto-oncogene products, such as the transcription factor c-Jun. The HAP1 protein is a candidate for pathology associated with HD.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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HAP1 gene expression is associated with radiosensitivity in breast cancer cells
Wu J, et al.
Biochemical and Biophysical Research Communications, 456(1), 162-166 (2015)
Lack of huntingtin-associated protein-1 causes neuronal death resembling hypothalamic degeneration in Huntington's disease
Li S H, et al.
The Journal of Neuroscience, 23(17), 6956-6964 (2003)
Interaction of huntingtin-associated protein with dynactin P150Glued
Li S H, et al.
The Journal of Neuroscience, 18(4), 1261-1269 (1998)
Huntingtin and huntingtin-associated protein 1 influence neuronal calcium signaling mediated by inositol-(1, 4, 5) triphosphate receptor type 1
Tang T S, et al.
Neuron, 39(2), 227-239 (2003)
Huntingtin-associated protein-1 interacts with pro-brain-derived neurotrophic factor and mediates its transport and release
Wu L, et al.
The Journal of Biological Chemistry, 285(8), 5614-5623 (2010)
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