SAB4200532

Anti-Villin-1 (C-terminal region) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-VIL, Anti-VIL1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~95 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 20-30 μg/mL using human small intestine.; immunoprecipitation (IP): 5-10 μg using Caco-2 cells.; indirect immunofluorescence: 5-10 μg/mL using Caco-2 cells.; western blot: 1-2 μg/mL using WiDR cells.
• UniProt accession no.: P09327
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... VIL1(7429)

Description

General description

Villin also known as villin-1 or VIL1 is a Ca²⁺-regulated actin-binding protein and belongs to the gelsolin family. It is localized in the apical cytoplasm and brush borders of epithelial cells of the small and large intestinal mucosa, intrahepatic bile ducts, kidney proximal tubules, gall bladder epithelium and pancreatic ducts. Villin is located on the human chromosome at 2q35.

Specificity

Anti-Villin-1 (C-terminal region) specifically recognizes human villin-1.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human villin-1, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse villin-1.

application

Anti-Villin-1 (C-terminal region) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions

Villin-1 is involved in modulating the structure and assembly of actin filaments. It also functions in regulating cell migration through its phosphorylation at Tyr-60, Tyr-81 and Tyr-256. Villin is involved in microvillus assembly and acts as a marker for epithelial cell differentiation. Its expression is stimulated during intestinal metaplasia observed in Barrett′s esophagus and chronic atrophic gastritis. Overexpression of villin is seen in human adenocarcinomas, such as colorectal cancer.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable