SAB4200525

Anti-Kindlin-2 antibody, Mouse monoclonal

clone 3A3, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-KIND2, Monoclonal Anti-Kindlin-2 antibody produced in mouse, Monoclonal Anti-MIG2, Monoclonal Anti-Mitogen-inducible gene 2 protein, Monoclonal Anti-PH domain-containing family C member 1, Monoclonal Anti-PLEKHC1, Monoclonal Anti-UNC112, Monoclonal Anti-UNC112B, Monoclonal Anti-family C (with FERM domain) member 1, Monoclonal Anti-fermitin family member 2, Monoclonal Anti-mig-2, Monoclonal Anti-mitogen inducible gene 2 protein, Monoclonal Anti-pleckstrin homology domain containing
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 3A3, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~72 kDa
• species reactivity: bovine, monkey, human, mouse, rat, dog
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1.0-2.0 μg/mL using HeLa total cell extracts
• isotype: IgG1
• UniProt accession no.: Q96AC1
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FERMT2(10979) ; mouse ... Fermt2(218952) ; rat ... Fermt2(289992)

Description

General description

Monoclonal Anti-Kindlin-2 (mouse IgG1 isotype) is derived from the hybridoma 3A3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Kindlin-2 belongs to the Kindlin family of focal adhesion proteins. It is localized in all solid tissues of mesenchymal origin. Kindlin-2 consists of band 4.1 protein, ezrin, radixin and moesin (FERM) and pleckstrin homology (PH) domains. It is mapped on the human chromosome at 14q22.1.

Specificity

Monoclonal Anti-Kindlin-2 recognizes human, monkey, bovine, dog rat and mouse kindlin-2.

Immunogen

recombinant C-terminal region fusion protein of human Kindlin-2.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-Kindlin-2 antibody produced in mouse may be used in:

• immunoblotting
• flow cytometry
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry

Biochem/physiol Actions

Kindlin-2 plays an important role in integrin-mediated cell-extracellular matrix (ECM) adhesion and invasion. It is also involved in wound healing, angiogenesis and embryonic development. Deficiency of kindlin-2 leads to the lethality of embryos. Kindlin-2 expression can be altered by malignant transformations such as breast cancer and leiomyomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable