SAB4200524

Anti-NF1 antibody, Mouse monoclonal

clone NF1-4, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-NF1 antibody produced in mouse, Monoclonal Anti-NFNS, Monoclonal Anti-Neurofibromatosis-related protein NF-1, Monoclonal Anti-VRNF, Monoclonal Anti-WSS, Monoclonal Anti-neurofibromin 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: NF1-4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~250 kDa
• species reactivity: mouse, human
• concentration: ~2.0 mg/mL
• technique(s): western blot: 4-8 μg/mL using SW-48 total cell extracts.
• isotype: IgG1
• UniProt accession no.: P21359
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NF1(4763) ; mouse ... Nf1(18015)

Description

General description

Monoclonal Anti-NF1 (mouse IgG1 isotype) is derived from the hybridoma NF1-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Neurofibromin (NF1) is a cytoplasmic protein. It is localized in neurons, oligodendrocytes, Schwann cells, astrocytes and leukocytes. NF1 possesses two functional domains, such as Ras GTPase-activating proteins (RasGAP) and Sec14.

NF1 (neurofibromin 1) gene is mapped to human chromosome 17q11.2. The encoded protein is composed of more than 2800 amino acids.

Specificity

Monoclonal Anti-NF1 recognizes human, monkey, dog, and mouse NF1.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human NF1, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-NF1 antibody produced in mouse is suitable for immunoblotting and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.

Biochem/physiol Actions

NF1 (neurofibromin 1) is a tumor suppressor gene and negatively regulates RAS (proto-oncogene, GTPase). Mutation in NF1 is strongly associated with the pathogenesis of melanoma and also causes neurofibromatosis type 1. Loss of NF1 allele results in benign neurofibromas in non-myelinating Schwann cell and is associated with desmoplastic melanoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable