SAB4200523

Anti-OTUB1 antibody, Mouse monoclonal

clone OTU14, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-OTB1, Monoclonal Anti-OTU domain, Monoclonal Anti-OTU1, Monoclonal Anti-OTUB1 antibody produced in mouse, Monoclonal Anti-Ubiquitin-specific-processing protease OTUB1, Monoclonal Anti-otubain-1, Monoclonal Anti-ubiquitin aldehyde binding 1, Monoclonal Anti-ubiquitin-specific protease otubain 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: OTU14, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~31 kDa
• species reactivity: human
• concentration: ~2.0 mg/mL
• technique(s): western blot: 10-20 μg/mL using whole extracts of HEK-293 cells.
• isotype: IgG1
• UniProt accession no.: Q96FW1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... OTUB1(55611)

Description

General description

Monoclonal Anti-OTUB1 (mouse IgG1 isotype) is derived from the hybridoma OTU14 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. OTU domain, ubiquitin aldehyde binding 1 (OTUB1) is a cysteine protease. It exists in two isoforms obtained by alternative splicing. OTUB1 belongs to the ovarian-tumor-domain-containing proteases (OTU) family of deubiquitinating enzymes. It is located on the human chromosome at 11q13.1.

Specificity

Monoclonal Anti-OTUB1 recognizes human OTUB1.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminus of human OTUB1, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-OTUB1 antibody produced in mouse may be used in immunoblotting and immunocytochemistry.

Biochem/physiol Actions

OTU domain, ubiquitin aldehyde binding 1 (OTUB1) is implicated in mediating lymphocyte antigen responsiveness by stabilizing lymphocyte specific E3 ligase gene related to anergy in lymphocytes (GRAIL) in CD4⁺ T lymphocytes. OTUB1 is also found in Lewy bodies of the brain and it might be involved in the pathogenesis of neurodegenerative disorders. Upregulation of OTUB1 in mesangial cells (MC) play a role in decreasing decorin levels, thus affecting glomerulonephritides. Overexpression of OTUB1 is associated with hyperactivation of mitogen-activated protein kinase (MAPK) signaling and promote tumorigenic transformation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable