SAB4200499

Monoclonal Anti-NF1 antibody produced in mouse

clone NF1-333, tissue culture supernatant

• Synonym(s): Anti-DKFZp686J1293, Anti-NFNS, Anti-VRNF, Anti-WSS, Anti-neurofibromin 1 (neurofibromatosis, von Recklinghausen disease, Watson disease)
• MDL number: MFCD02264160
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: tissue culture supernatant
• antibody product type: primary antibodies
• clone: NF1-333, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~250 kDa
• species reactivity: rat, human, mouse
• technique(s): flow cytometry: 1:25-1:50/test using A549 cells.; western blot: 1:1000-1:2000 using Caco-2 total cell extracts.
• isotype: IgM
• UniProt accession no.: P21359
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NF1(4763) ; mouse ... Nf1(18015) ; rat ... Nf1(24592)

Description

General description

Monoclonal Anti-NF1 (mouse IgM isotype) is derived from the hybridoma NF1-333 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Neurofibromin 1 (NF1) gene is located at chromosome 17q11.2. It is predominantly expressed in neurons, Schwann cells, oligodendrocytes, astrocytes and leukocytes. NF1 has two potentially functional domains; Ras GTPase activating protein (RasGAP)and phosphatidylinositol-transfer protein.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminus of human NF1, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-NF1 antibody produced in mouse has been used in western blotting. It may be suitable for use in flow cytometry.

Biochem/physiol Actions

NF1 acts as a negative regulator of the p21-Ras/mitogen-activated protein kinase (MAPK) pathway as well as a tumor suppressor gene. Mutations in the neurofibromin 1 (NF1) gene is implicated in Neurofibromatosis type 1 (NF1) gene also known as von Recklinghausen peripheral or classical neurofibromatosis, is one of the most common autosomal dominant diseases. Consistent with a central role for neurofibromin in cellular function, recent cancer genome sequencing studies have found that somatic NF1 gene mutations occur not only in association with NF1, but also in a number of other common cancers. A variety of benign and malignant tumours are associated with NF1 and all involve tumorigenesis of neural crest-derived cells.

Physical form

Culture supernatant solution containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable