SAB4200498

Monoclonal Anti-SET antibody produced in mouse

clone SET7, tissue culture supernatant

• Synonym(s): Monoclonal Anti-2PP2A, Monoclonal Anti-I2PP2A, Monoclonal Anti-IGAAD, Monoclonal Anti-IPP2A2, Monoclonal Anti-PHAPII, Monoclonal Anti-SET nuclear oncogene, Monoclonal Anti-TAF-I, Monoclonal Anti-TAF-IBETA
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: tissue culture supernatant
• antibody product type: primary antibodies
• clone: SET7, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~37 kDa
• species reactivity: human
• technique(s): flow cytometry: 1:25-1:50/test using A549 cells; western blot: 1:2000-1:4000 using A549 total cell extracts
• isotype: IgA
• UniProt accession no.: Q01105
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SET(6418)

Description

General description

Monoclonal Anti-SET (mouse IgA isotype) is derived from the hybridoma SET7 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. The SET nuclear proto-oncogene (SET7) protein localizes both to the nucleus and the cytoplasm.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminus of human SET, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-SET antibody produced in mouse has been used in immunoblotting and flow cytometry.

Biochem/physiol Actions

SET nuclear proto-oncogene (SET7) is involved in the regulation of signal transduction in normal and cancer cells. It contains a highly acidic C-terminal region that is involved in chromatin remodeling. In fact, SET itself is also a potent and specific inhibitor of protein phosphatase 2A (PP2A) activity and exerts its activity via its N-terminal region. SET also acts as an inhibitor of the tumor suppressor NM23-H1, which is activated by granzyme A DNAse and negatively controls histone acetylation. Interestingly, in acute non-lymphocytic myeloid leukemia, SET was found to be fused to CAN (nucleoporin Nup214), apparently as a result of a chromosomal translocation. As such, the formation of this SET-CAN fusion protein may impair the normal regulation of PP2A and contribute to leukemogenesis. SET was also found to regulate human NK-cell cytotoxicity, by regulating NK-cell IFN-g production and granzyme B gene expression via PP2A inactivation.

Physical form

Solution containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable