SAB4200496

Anti-LMOD1 antibody, Mouse monoclonal

clone LMOD1-12, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-LMOD1 antibody produced in mouse, Monoclonal Anti-SM-LMOD, Monoclonal Anti-leiomodin 1 (smooth muscle)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: LMOD1-12, monoclonal
• form: buffered aqueous solution
• mol wt: ~70 kDa
• species reactivity: human, mouse, rat
• concentration: ~1.0 mg/mL
• technique(s): western blot: 0.1-0.2 μg/mL using whole extracts of HEK-293T cells overexpressing LMOD1.
• isotype: IgG2a
• UniProt accession no.: P29536
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LMOD1(25802)

Description

General description

Monoclonal Anti-LMOD1 (mouse IgG2a isotype) is derived from the hybridoma LMOD1-12 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Leiomodin 1 (LMOD1) shares its homology greatly with tropomodulin, a tropomyosin-binding, actin-capping protein. Leiomodin contains two actin-binding sites, a flexible N-terminal domain with a tropomyosin-binding site and the leucine-rich repeat domain. It also has a C-terminal domain with a short polyproline region and an actin-binding Wiskott-Aldrich syndrome protein (WASP)-homology 2 (WH2) domain. It is expressed predominantly in smooth muscle. The LMOD1 gene is mapped to the human chromosome location 1q32.1.

Specificity

Monoclonal Anti-LMOD1 recognizes human and rat LMOD1.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminal region of human LMOD1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine and canine LMOD1.

Application

Monoclonal Anti-LMOD1 antibody produced in mouse may be used in immunoprecipitation and immunoblotting.

Biochem/physiol Actions

Leiomodin 1 (LMOD1) is an actin filament nucleator in muscle cells. It is found near the pointed ends of the actin filaments and is involved in sarcomere assembly and organization. LMOD1 knockdown results in sarcomere disruption. Overexpression of LMOD1 may be associated with Graves′ disease and thyroid-associated ophthalmopathy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable