immunohistochemistry: 1:500-1:1,000 using formalin-fixed, paraffin-embedded human small intestine. indirect ELISA: 1:10,000-1:20,000 using human adrenomedullin.
Adrenomedullin is isolated from human pheochromocytoma. It is highly expressed in endothelial cells and vascular smooth muscle cells and is localized in highly vascularized tissues including kidney, intestine, stomach, brain, lung, heart and placenta.
Immunogen
synthetic peptide corresponding to a sequence at the N-terminus of human adrenomedullin (ADM), conjugated to KLH. The corresponding sequence has significant homology (75% sequence identity) in rat and mouse ADM.
Application
Anti-Adrenomedullin (N-terminal) antibody produced in rabbit has been used in immunohistochemistry and enzyme linked immunosorbent assay (ELISA).
Biochem/physiol Actions
Adrenomedullin (ADM, AM) is a potent hypotensive peptide. In addition to its vasodilator effect, ADM also exerts a wide range of biological actions including diuretic and cardiotonic effects. ADM is involved in the regulation of hormone release, inflammation, oxidative stress as well as the proliferation, migration and differentiation of various cell types. ADM is thought to play a significant role in angiogenesis and in pathological conditions including cardiovascular disease, ischemia and carcinogenesis. ADM is broadly distributed in many tissues during development and in adulthood. ADM exerts its effects via G-protein coupled calcitonin-like receptors (CRLR) complexed with either receptor activity-modifying protein-2 (RAMP2) or RAMP3.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Receptor Activity-Modifying Proteins: RAMPing up Adrenomedullin Signaling
Gibbons C, et al.
Molecular Endocrinology, 21(4), 783-796 (2007)
Immunohistological localization and possible functions of adrenomedullin
Marutsuka K, et al.
Hypertension Research, 26(Suppl), S33-S40 (2003)
Distribution of adrenomedullin-like immunoreactivity in the rat central nervous system by light and electron microscopy
Serrano J, et al.
Brain Research, 853(2), 245-268 (2000)
The GPCR modulator protein RAMP2 is essential for angiogenesis and vascular integrity
Ichikawa SY, et al.
The Journal of Clinical Investigation, 118(1), 29-39 (2008)
A review of the biological properties and clinical implications of adrenomedullin and proadrenomedullin N-terminal 20 peptide (PAMP), hypotensive and vasodilating peptides
Et T.
Peptides, 22(11), 1693-1711 (2001)
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