SAB4200487

Anti-RASD1

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-AGS1, Anti-DEXRAS1, Anti-Dexamethasone-induced Ras-related protein 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~32 kDa
• species reactivity: human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using cell lysates of HEK-293T overexpressing human RASD1
• UniProt accession no.: Q9Y272
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RASD1(51655) ; mouse ... Rasd1(19416) ; rat ... Rasd1(64455)

Description

General description

Ras-related dexamethasone-induced 1 (RASD1) belongs to the Ras family of monomeric G-proteins and was initially recognized as a dexamethasone inducible gene. RASD1 comprises an effector loop, four highly conserved guanosine-5′-triphosphate (GTP) binding and hydrolysis pockets (Σ1-Σ4). The Σ1 and Σ2 pockets have phosphate-magnesium binding domains and the Σ3 and Σ4 comprises guanine nucleotide binding loops. The C-terminal region of RASD1 includes a cationic CAAX-domain. RASD1 is expressed in the liver, heart, white adipose tissues and kidneys. The RASD1 gene is present at the human chromosome location 17p11.2.

Specificity

Anti-RASD1 specifically recognizes human RASD1.

Immunogen

synthetic peptide corresponding to an internal region of human RASD1, conjugated to KLH. The corresponding sequence is identical in mouse and rat RASD1.

Application

Anti-RASD1 is suitable for use in immunoblotting.

Biochem/physiol Actions

Ras-related dexamethasone-induced 1 (RASD1) /Dexras1 modulates various signaling pathways like, N-methyl-d-aspartic acid receptors (NMDAR)-nitric oxide, G-protein coupled receptors (GPCRs), mitogen-activated protein kinase/extracellular signal-regulated protein kinase (MAPK/ERK) and protein kinase C (PKC). Dexras1 functions as a novel physiological target of neuronal NO synthase (nNOS). The development of a ternary complex between nNOS, the nNOS adaptor protein CAPON and DexRAS1 promotes the ability of nNOS to activate Dexras1. It is S-nitrosylated, which is activated by nitric oxide (NO) donors and by NMDA receptor-stimulated NO-synthesis in cortical neurons. Knock-out of nNOS in mice results in the selective reduction of Dexras1 activation. NMDA receptor-enhanced NO-signaling mediates neuronal iron homeostasis through Dexras1. An iron-uptake signaling cascade including NMDA-NO-Dexras1-peripheral benzodiazepine receptor associated protein (PAP7)-divalent metal transporter 1 (DMT1) facilitates NMDA neurotoxicity. Dexras1 interacts with FE65 and directly regulates FE65-amyloid precursor protein (APP)-mediated transcription.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable