SAB4200486

Anti-Dysbindin (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-DBND, Anti-DTNBP1, Anti-HPS7, Anti-My031, Anti-SDY
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: human, rat
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 10-20 μg using extracts of HEK-293T cells overexpressing human dysbindin (DTNBP1).; indirect immunofluorescence: 5-10 μg/mL using PC12 cells.; western blot: 0.5-1 μg/mL using extracts of HEK-293T cells overexpressing human dysbindin (DTNBP1).
• UniProt accession no.: Q96EV8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DTNBP1(84062) ; rat ... Dtnbp1(641528)

Description

General description

Dysbindin-1 (DTNBP1) is a protein, encoded by the dystrobrevin-binding protein 1 gene. Initially, it was identified to be a constituent of the dystrophin-associated protein complex (DPC) in skeletal muscle cells. The DTNBP1 protein consists of two coiled-coil domains and it exists in three isoforms, namely dysbindin-1A, -1B and -1C. DTNBP1 mRNA is widely expressed in the brain, located in postsynaptic densities and synaptic vesicles of neurons. The gene is present at the human chromosome location 6p22.3.

Specificity

Anti-Dysbindin(C-terminal) specifically recognizes human and rat dysbindin.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human dysbindin, conjugated to KLH. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Application

Anti-Dysbindin (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Dysbindin (also known as DTNBP1) is a neuronal protein that controls synaptic homeostasis by regulating the exocytosis and formation of synaptic vesicles. Polymorphism of DTNBP1 confers susceptibility to schizophrenia through a decreased expression of dysbindin, especially in the hippocampal formation. Deletion mutation of DTNBP1 in sandy (sdy) mouse is linked to impaired long term memory retention and working memory. Mutations in the human DTNBP1 gene are associated with Hermansky-Pudlak syndrome (HPS). Dysbindin is a key component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1), which regulates the trafficking of proteins in the lysosomal pathway. At postsynaptic densities, it is involved in trafficking and tethering of receptors and signal transduction proteins.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable