SAB4200482

Anti-ADAR1 antibody, Mouse monoclonal

clone ADAR61, purified from hybridoma cell culture

• Synonym(s): Anti-ADAR1, Anti-DRADA, Anti-DSH, Anti-DSRAD, Anti-G1P1, Anti-IFI4, Anti-K88DSRBP, Anti-P136, Monoclonal Anti-ADAR1 antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: ADAR61, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 80-130 kDa
• species reactivity: monkey, human, rat
• concentration: ~1.0 mg/mL
• technique(s): flow cytometry: suitable; immunoprecipitation (IP): suitable; western blot: 2.0-4.0 μg/mL using U2OS total cell extracts
• isotype: IgG3
• UniProt accession no.: P55265
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ADAR(103)

Description

General description

Monoclonal Anti-ADAR1 (mouse IgG3 isotype) is derived from the hybridoma ADAR61 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Adenosine deaminase that acts in RNA 1 (ADAR1) and ADAR2 are expressed in most tissues but in general, the pre-mRNA being edited represents receptors of the central nervous systems. ADAR1 has several isoforms which include ADAR1 (150kDa) and two short isoforms (80 and 110 kDa) that are functional.

Immunogen

a peptide corresponding to a sequence at the C-terminus of human ADAR, conjugated to KLH.

Application

Monoclonal Anti-ADAR1 antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• flow cytometry

Biochem/physiol Actions

RNA editing by hydrolytic deamination of adenosine (A) to inosine (I) in double-stranded (ds)RNA is the most common type of editing in higher eukaryotes. This RNA editing event is catalyzed by the adenosine deaminase that acts in RNA (ADAR) enzyme. Two ADAR enzymes have been shown to possess enzymatic activity in mammals, ADAR1 and ADAR2. Both can convert specific adenosine to inosine in pre-mRNA and can also convert up to 40-50% of the adenosines in long synthetic duplex RNAs. ADAR1 plays critical roles in differentiating cells of embryo and adult tissues, supporting cell survival and permitting their further differentiation and maturation. Its role in embryonic development is especially demonstrated within the hematopoietic lineage as well as in adult hematopoietic progenitor cells (HPCs). ADAR1 is also an essential regulator of hematopoietic stem cell maintenance and suppressor of interferon signaling that may protect organisms from the deleterious effects of interferon activation associated with many pathological processes, including chronic inflammation, autoimmune disorders and cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable