SAB4200480

Anti-Kindlin-1 antibody, Mouse monoclonal

clone 4A5.14, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-C20orf42, Monoclonal Anti-DTGCU2, Monoclonal Anti-FERMT1 URP1, Monoclonal Anti-KIND1, Monoclonal Anti-Kindlin-1 antibody produced in mouse, Monoclonal Anti-UNC112A, Monoclonal Anti-fermitin family member 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 4A5.14, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~77 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: 2.5-5.0 μg using SW-48 cells; immunoprecipitation (IP): suitable; western blot: 2.0-4.0 μg/mL using SW-48 total cell extracts
• isotype: IgG2a
• UniProt accession no.: Q9BQL6
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FERMT1(55612)

Description

General description

Monoclonal Anti-Kindlin-1 (mouse IgG2a isotype) is derived from the hybridoma 4A5.14 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Kindlin-1 is a focal adhesion protein, which belongs to the family of Kindlin proteins. It is highly expressed in epithelial cells, including keratinocytes. Kindlin-1 is localized to the focal adhesions. A splice variant potentially producing a truncated protein has been detected in the kidney, colon and small intestine. Kindlin-1 comprises a bipartite 4.1 protein, ezrin, radixin, moesin (FERM) domain with four subdomains (F0, F1, F2, and F3), which is found in many proteins. The KIND1 gene is present at human chromosome location 20p12.3.

Specificity

Monoclonal Anti-Kindlin-1 recognizes human Kindlin-1.

Immunogen

human Kindlin-1 recombinant fusion protein.

Application

Monoclonal Anti-Kindlin-1 antibody produced in mouse may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry
• flow cytometry

Biochem/physiol Actions

Kindlin-1 is implicated in the activation of integrin adhesion receptors and the reorganization of the actin cytoskeleton. Deficiency of kindlin-1 is linked to Kindler syndrome, an autosomal recessive genodermatosis characterized by skin blistering, progressive skin atrophy, photosensitivity and carcinogenesis. This gene is also implicated in breast cancer, lung metastasis and lung tumorigenesis. The 4.1 protein, ezrin, radixin, moesin (FERM) domain of Kindlin-1 is involved in cytoskeletal organization. In colon cancer, Kindlin-1 is identified as a potential marker. Kindlin-1 modulates epidermal growth factor receptor signaling.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable