SAB4200474

Anti-DIS3 antibody, Mouse monoclonal

clone DIS3-3, purified from hybridoma cell culture

• Synonym(s): Anti-EXOSC11, Anti-KIAA1008, Anti-RP11-342J4.3, Anti-RRP44, Anti-dis3p, Monoclonal Anti-DIS3 antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: DIS3-3, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~109 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): suitable; indirect immunofluorescence: 2.5-5.0 μg/mL using human HeLa cells; western blot: 1-2 μg/mL using whole extracts of human HEK-293T cells
• isotype: IgG1
• UniProt accession no.: Q9Y2L1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DIS3(22894)

Description

General description

Monoclonal Anti-DIS3 (mouse IgG1 isotype) is derived from the hybridoma DIS3-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human DIS3, conjugated to keyhole limpet hemocyanin (KLH). DIS3 homolog, exosome endoribonuclease and 3′-5′ exoribonuclease (DIS3) mitotic control homolog is a catalytic component of the eukaryotic RNA exosome. The RNA exosome is a multi-subunit complex with ribonucleolytic complex of 400 kDa and nine subunit which forms the catalytically inactive core. The exosome complex is localized to the nucleus and the cytoplasm. DIS3 is mainly nuclear, whereas DIS3L is confined to cytoplasm.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human DIS3, conjugated to KLH.

Application

Monoclonal Anti-DIS3 antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

DIS3 homolog, exosome endoribonuclease and 3′-5′ exoribonuclease (DIS3) is involved in RNA processing and turnover. Its core subunit maintains structural function and is involved in substrate recognition. The exosome is important for the 3′ → 5′ processing and degradation of many types of RNAs including mRNA, rRNA, snRNA, snoRNA and tRNA. DIS3 and DIS3L are active exonucleases. DIS3 also displays endonuclease activity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable