immunoprecipitation (IP): 1-2 μg using lysates of rat NRK cells. indirect immunofluorescence: 2.5-5.0 μg/mL using human HeLa cells western blot: 0.25-0.5 μg/mL using whole extracts of mouse NIH-3T3 cells.
Staphylococcal nuclease and tudor domain containing 1 (SND1) protein contains five staphylococcal nuclease-like domains and a tudor-like domain. SND1 is a component of the RNA-induced silencing complex (RISC). This gene is highly conserved from yeast to human.
Immunogen
peptide corresponding to the C-terminal region of human SND1 (GeneID: 27044), conjugated to KLH. The corresponding sequence is identical in mouse and rat.
Application
Anti-SND1 (C-terminal) antibody produced in rabbit has been used in immunoblotting, immunoprecipitation and immunofluorescence.
Biochem/physiol Actions
Staphylococcal nuclease and tudor domain containing 1 (SND1) has been shown to be involved in the regulation of transcription and RNA biogenesis. It is known to interact with nucleic acids and proteins. SND1 promotes the breakdown of hyper-edited inosine-containing miRNA precursors. It modulates miRNA transcription and translation through RNA editing by ADAR (adenosine deaminase acting on RNA). Increased SND1 expression is seen in human colon cancer tissues and cell lines.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Colon cancers have been shown to develop after accumulation of multiple genetic and epigenetic alterations with changes in global gene expression profiles, contributing to the establishment of widely diverse phenotypes. Transcriptional and posttranscriptional regulation of gene expression by small RNA
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