SAB4200469

Anti-POLR2A antibody produced in rabbit

Ig fraction of antiserum

• Synonym(s): Anti-POLR2, Anti-POLRA, Anti-RPBh1, Anti-RPO2, Anti-RPOL2, Anti-RpIILS, Anti-hRPB220, Anti-hsRPB1, Anti-polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RPB1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: Ig fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~250 kDa
• species reactivity: human
• technique(s): immunoprecipitation (IP): 5-10 μL using whole extract of human HeLa cells; indirect immunofluorescence: 1:400-1:800 using human HeLa cells; western blot: 1:2000-1:4000 using nuclear extracts of HeLa cells
• UniProt accession no.: P24928
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... POLR2A(5430) ; mouse ... Polr2a(20020) ; rat ... Polr2a(363633)

Description

General description

RNA polymerase II subunit A (POLR2A) gene with 29 exons is mapped on human chromosome 17p13.1. This gene codes for a catalytic subunit of RNA polymerase II. The encoded protein consists of a highly conserved 5′ flanking region.

Immunogen

peptide corresponding to the N-terminal region of human POLR2A, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine, dog and pig.

Biochem/physiol Actions

RNA polymerase II subunit A (POLR2A) catalyzes the synthesis of messenger RNA in eukaryotes. The 5′ flanking domain of RNA polymerase II subunit A (POLR2A) plays a vital role in transcriptional regulation. Mutation in the gene is associated with the pathogenesis of meningiomas.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable