SAB4200467

Anti-Migfilin antibody, Mouse monoclonal

clone 43, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-CAL, Monoclonal Anti-FBLIM1, Monoclonal Anti-FBLP-1, Monoclonal Anti-FBLP1, Monoclonal Anti-Migfilin antibody produced in mouse, Monoclonal Anti-filamin binding LIM protein 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 43, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 41 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 20 μg/mL using A431 cells.; western blot: 0.1-0.2 μg/mL using A431 total cell extracts.
• isotype: IgG1
• UniProt accession no.: Q8WUP2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FBLIM1(54751)

Description

General description

FBLIM1 (filamin binding LIM protein 1) is present at the cell-cell adhesion areas. The protein has the amino-terminal, proline rich-region and carboxy-terminal LIM (LIN-11, ISL-1 and MEC-3) domains.[1] It is commonly referred to as migfilin.

Monoclonal Anti-Migfilin (mouse IgG1 isotype) is derived from the hybridoma 43 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. The Migfilin gene is mapped to the human chromosome location 1p36.21.

Specificity

Monoclonal Anti-Migfilin recognizes human migfilin.

Immunogen

recombinant human migfilin, fusion protein.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-Migfilin antibody produced in mouse may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry

Biochem/physiol Actions

FBLIM1 (filamin binding LIM protein 1) plays an important role in association between the cell membrane and the actin cytoskeleton. It mainly interacts with MIG-2 (mitogen-inducible gene 2 protein), filamin and VASP (vasodilator-stimulated phosphoprotein), thus controlling cell shape and movements. In cardiomyocytes, it interacts with transcription factor CSX/NKX2-5 (cardiac-specific homeobox) and enhances the differentiation. The FBLIM1 gene is upregulated in osteoarthritis chondrocytes and might be involved with osteoarthritis pathogenesis. In various carcinoma cells, the FBLIM1-associated signaling in disrupted which leads to abnormal Src activation and anoikis resistance in cells. This gene is downregulated in breast cancer cells. In esophageal cancer cells, it suppresses invasion partly by enhancing degradation of β-catenin. However, in glioma cells FBLIM1 promotes migration as well as invasion.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable