SAB4200463
Anti-PICK1 (C-terminal) antibody produced in rabbit
IgG fraction of antiserum
Synonym(s):
Anti-PICK, Anti-PRKCABP, Anti-Protein kinase C-alpha-binding protein, Anti-protein interacting with PRKCa
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~47 kDa (Rat and Mouse PICK1)
antigen ~55 kDa (Human PICK1)
species reactivity
rat, human, mouse
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
western blot: 1:2000-1:4000 using rat brain extracts (S1 fraction), mouse brain extracts (S1 fraction) and lysates of HEK-293T cells overexpressing human PICK1.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PICK1(9463)
mouse ... Pick1(18693)
rat ... Pick1(84591)
General description
Protein interacting with C kinase 1 (PICK1) is a post-synaptic density-95/discs large/zonula occludens-1 (PDZ) and Bin/Ampiphysin/Rvs (BAR)-domain containing protein. PICK1 is expressed at high levels in the brain, testis and is located at the perinuclear region as well as synapses of neurons. The PICK1 gene is located on the human chromosome 22q13.1.
Specificity
Anti-PICK1(C-terminal) specifically recognizes human,rat and mouse PICK1.
Immunogen
synthetic peptide corresponding to a sequence at the C-terminus of rat PICK1, conjugated to KLH. The corresponding sequence is highly conserved in mouse PICK1 (single amino acid substitution) and in human PICK1 (83% sequence identity).
Application
Anti-PICK1 (C-terminal) antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
Protein interacting with C kinase 1 (PICK1) plays a central role in a specific form of vesicular trafficking, modulation of presynaptic glutamate neurotransmission by targeting and anchoring glutamate receptors to specific synapses and synaptic plasticity. PICK1 is phosphorylated in response to protein kinase C α (PKCα) activation. The PICK1 binds to a large number of membrane proteins, including the glutamate receptors subunits α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR), metabotropic glutamate receptor 7a (mGLUR7a) and the ephrin receptors to regulate their subcellular localization via the post-synaptic density-95/discs large/zonula occludens-1 (PDZ) domain. The Bin/Ampiphysin/Rvs (BAR) domain binds to lipids, mainly phosphoinositides, which are important for PICK1 regulation, synaptic targeting and trafficking of AMAP receptors. Mutations in the PICK1 PDZ domain inhibit the binding of PICK1 to PKCα.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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K Fujii et al.
Molecular psychiatry, 11(2), 150-157 (2005-11-30)
Accumulating evidence from both genetic and clinico-pharmacological studies suggests that D-serine, an endogenous coagonist to the NMDA subtype glutamate receptor, may be implicated in schizophrenia (SZ). Although an association of genes for D-serine degradation, such as D-amino acid oxidase and
H Boudin et al.
Neuron, 28(2), 485-497 (2001-01-06)
Aggregation of neurotransmitter receptors at pre- and postsynaptic structures is crucial for efficient neuronal communication. In contrast to the wealth of information about postsynaptic specializations, little is known about the molecular organization of presynaptic membrane proteins. We show here that
Jonathan G Hanley
Pharmacology & therapeutics, 118(1), 152-160 (2008-03-21)
AMPA (alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid) receptor trafficking is a fundamental mechanism for regulating synaptic strength, and hence may underlie cellular processes involved in learning and memory. PICK1 (protein interacting with C-kinase) is a PDZ and BAR domain-containing protein that has recently emerged
Wenying Jin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(9), 2380-2390 (2006-03-03)
The targeting and surface expression of membrane proteins are critical to their functions. In neurons, synaptic targeting and surface expression of AMPA-type glutamate receptors were found to be critical for synaptic plasticity such as long-term potentiation and long-term depression (LTD).
Junyu Xu et al.
Neuro-Signals, 15(4), 190-201 (2007-01-12)
PICK1 is a peripheral membrane protein conserved from Caenorhabditis elegans to the human. It is expressed in many tissues with high levels in brain and testis. Inside cells, PICK1 is localized at the perinuclear region as well as specialized structures
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