SAB4200448

Anti-Phospho-PRAS40 (pThr²⁴⁶) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-AKT1S1
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~40 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human breast carcinoma; indirect immunofluorescence: 10-20 μg/mL using HeLa cells; western blot: 1.5-3.0 μg/mL using HEK-293T cell extracts
• UniProt accession no.: Q96B36
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pThr246)
• Gene Information: human ... AKT1S1(84335) ; mouse ... Akt1s1(67605) ; rat ... Akt1s1(292887)

Description

General description

Proline-rich Akt substrate of 40 kilodalton (PRAS40), also known as AKT1 substrate 1, is encoded by the gene mapped to human chromosome 19q13.33.

Immunogen

synthetic phospho-peptide containing phosphorylated Thr²⁴⁶ of human PRAS40, conjugated to KLH. The corresponding sequence is identical in mouse and rat PRAS40.

Application

Anti-Phospho-PRAS40 (pThr246) antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Proline-rich Akt substrate of 40 kilodalton (PRAS40) acts as a linker between micro-environmental stress signaling and exosome-mediated secretion. The encoded protein might inhibit the activity of the mTOR complex 1 (mTORC1) kinase. Mutation in the gene is associated with the development of of cancers. Thus, this gene can be considered as a potential target for treatment of several cancers. PRAS40 plays a major role in the regulation of insulin sensitivity in human skeletal muscle cells (hSkMC).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable