SAB4200447

Monoclonal Anti-BTBD9 antibody produced in mouse

clone BTBD9-205, tissue culture supernatant

• Synonym(s): Anti-BTB (POZ) domain containing 9
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: tissue culture supernatant
• antibody product type: primary antibodies
• clone: BTBD9-205, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~70 kDa
• species reactivity: mouse, human, monkey, canine
• technique(s): indirect immunofluorescence: 1:500-1:1000 using HeLa cells; western blot: 1:8000-1:16,000 using extracts of K562 cells
• UniProt accession no.: Q96Q07
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BTBD9(114781)

Description

General description

Monoclonal Anti-BTBD9 (mouse IgM isotype) is derived from the hybridoma BTBD9-205 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to a sequence at the C-terminus of human BTBD9 conjugated to KLH protein, that contains one BTB/POZ domain and one BACK (BTB/Kelch associated) domain. The BTB/POZ domain mediates homomeric and heteromeric protein-protein interactions.

The BTBD9 gene is mapped to human chromosome 6p21.2.

Specificity

Monoclonal Anti-BTBD9 recognizes human, monkey, dog and mouse BTBD9.

Immunogen

a peptide corresponding to a sequence at the C-terminus of human BTBD9, conjugated to KLH.

Application

Monoclonal Anti-BTBD9 antibody produced in mouse has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

BTBD9 protein plays a vital role in protein-protein interactions.

Mutations in BTBD9 (BTB/POZ domain-containing protein 9) is associated with restless legs syndrome type 6 (RLS6) and Tourette syndrome.

Physical form

Culture supernatant solution containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 0C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable