SAB4200444

Anti-Aurka/Aurora-A Kinase antibody, Mouse monoclonal

~1.0 mg/mL, clone AR-7, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-AIK, Monoclonal Anti-ARK1, Monoclonal Anti-AURA, Monoclonal Anti-AURORA2, Monoclonal Anti-Aurka/Aurora-A Kinase antibody produced in mouse, Monoclonal Anti-BTAK, Monoclonal Anti-PPP1R47, Monoclonal Anti-STK15, Monoclonal Anti-STK6, Monoclonal Anti-STK7
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: AR-7, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~46 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5.0 μg/mL using HT-29 cells; western blot: 2-4 μg/mL using extracts of THP-1 cells
• isotype: IgG1
• UniProt accession no.: O14965
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... AURKA(6790)

Description

General description

Monoclonal Anti-Aurka/Aurora-A Kinase (mouse IgG1 isotype) is derived from the hybridoma AR-7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Aurka/Aurora-A Kinase is a member of serine/threonine kinase family. The AURKA gene is mapped on the human chromosome at 20q13.2.

Specificity

Monoclonal Anti-Aurka/Aurora-A Kinase recognizes human and monkey Aurka.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human Aurka, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-Aurka/Aurora-A Kinase antibody produced in mouse may be used in immunoblotting and immunocytochemistry.

Biochem/physiol Actions

Aurka/Aurora-A Kinase is involved in correct centrosomal and microtubule functions. It is also involved in the chromosomal segregation during normal cellular mitosis. This protein plays a role in the recruitment of cyclin-dependent kinase (CDK)-cyclin B1 to the centrosome, which correlates with its activation and the fidelity of the cells to mitosis. Ectopic expression and altered activity of the AURKA gene are observed to induce centrosome amplification leading to genomic instability, aneuploidy and abnormal mitosis. Overexpression of the AURKA gene is observed in a spectrum of cancers such as colorectal, breast and gastric cancers. Overexpression of this gene is also associated with high-grade tumors, invasion and metastasis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable