SAB4200443

Anti-ELMO1 antibody, Mouse monoclonal

clone ELMO1-6, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-CED-12, Monoclonal Anti-CED12, Monoclonal Anti-ELMO-1, Monoclonal Anti-ELMO1 antibody produced in mouse, Monoclonal Anti-Engulfmnet and cell motility 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: ELMO1-6, monoclonal
• mol wt: antigen ~75 kDa
• species reactivity: human, mouse, rat
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using whole extracts of human Raji or mouse A20 cells.
• isotype: IgG1
• UniProt accession no.: Q92556
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ELMO1(9844) ; mouse ... Elmo1(140580) ; rat ... Elmo1(361251)

Description

General description

Monoclonal Anti-ELMO1 (mouse IgG1 isotype) is derived from the hybridoma ELMO1-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Engulfment and cell motility 1 (ELMO1) protein is a member of the evolutionarily conserved family of ELMO proteins. The ELMO1 gene is mapped on the human chromosome at 7p14.2-p14.1.

Specificity

Monoclonal Anti-ELMO1 recognizes human and mouse ELMO1.

Immunogen

synthetic peptide corresponding to a sequence near the C-terminus of human ELMO1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, bovine, monkey and dog and differs by 4 amino acids in ELMO2.

Application

Monoclonal Anti-ELMO1 antibody produced in mouse may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Engulfment and cell motility (ELMO) regulates the actin cytoskeleton reorganization during engulfment and cell migration. ELMO along with the dedicator of cytokinesis (Dock)-180 function as a guanine exchange factor (GEF) for the small guanosine triphosphatase (GTPase) Rac, leading to its activation and cytoskeleton rearrangement. ELMO1 interacts with brain-specific angiogenesis inhibitor 1 (BAI1), an engulfment receptor for apoptotic cells upstream of the ELMO1/Dock180/Rac module. High expression levels of ELMO1 and Dock180 are linked to the invasive phenotype of glioma cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable