SAB4200437

Anti-EXOSC1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-CGI-108, Anti-CSL4, Anti-Csl4p, Anti-Exosome component 1, p13, Anti-SKI4, Anti-Ski4p, Anti-hCsl4p
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse, human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 1-2 μg/mL using human HeLa cells.; western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human tagged-EXOSC1 (~26 kDa).
• UniProt accession no.: Q9Y3B2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EXOSC1(51013) ; mouse ... Exosc1(66583)

Description

General description

The EXOSC1 gene is located on human chromosome 10q24.1.

The gene EXOSC1 (exosome component 1) encodes one of the nine core subunits of the exosome complex. The protein is also referred to as Csl4 and has high affinity for RNA.

Specificity

Anti-EXOSC1 recognizes human EXOSC1.

Immunogen

peptide corresponding to a sequence at the N-terminal region of human EXOSC1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine and dog.

Application

Anti-EXOSC1 antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

The exosome complex facilitates the splicing and degradation of mRNAs and non-coding RNAs. It is also essential for epigenetic gene regulation. It endogenously suppresses erythroid maturation. Within the complex, Exosc1 (exosome component 1) associates with Exosc6 to stabilize the complex. The binding of Exosc10 to Exosc1, Exosc6, and Exosc8 enhances the activity of the complex and positions Exosc1 for RNA binding.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable