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SAB4200435

Anti-COG7 antibody produced in rabbit

Name: Anti-COG7 antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-CDG2E, Anti-Component of oligomeric golgi complex 7

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~86 kDa

species reactivity

mouse, rat, human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells.
indirect immunofluorescence: 1-2 μg/mL using human HeLa cells.
western blot: 2.5-5.0 μg/mL using whole extracts of mouse LA-4 and rat PC12 cells.

UniProt accession no.

P83436

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... COG7(91949)
mouse ... Cog7(233824)
rat ... Cog7(293456)

General description

Conserved Oligomeric Golgi complex 7 (COG7) protein belongs to the COG complex. COG complex is composed of eight distinct subunits organized in two heterotrimeric groups, COG2, 3, 4 and COG5, 6, 7 which are linked via a dimeric group formed by COG1 and COG8. COG complex is an evolutionarily conserved multi-subunit protein complex. The COG7 gene is located on the human chromosome at 16p12.2.

Specificity

Anti-COG7 recognizes human, mouse and rat COG7.

Immunogen

peptide corresponding to an internal region of human COG7, conjugated to KLH. The corresponding sequence is identical in mouse and rat COG7.

Application

Anti-COG7 antibody produced in rabbit may be used in:

immunoblotting
immunoprecipitation
immunofluorescence

Biochem/physiol Actions

Conserved Oligomeric Golgi complex (COG) modulates membrane trafficking and maintenance of Golgi glycosylation machinery in eukaryotic cells. COG7 protein is involved in retrograde vesicular trafficking. Mutations in the COG7 gene leads to type IIe congenital disorder of glycosylation.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A new mutation in COG7 extends the spectrum of COG subunit deficiencies.

Renate Zeevaert et al.

European journal of medical genetics, 52(5), 303-305 (2009-07-07)

We describe a patient homozygous for a novel mutation in COG7, coding for one of the subunits of the Conserved Oligomeric Golgi complex, involved in retrograde vesicular trafficking. His brother showed a similar clinical syndrome and glycosylation defect but no

A common mutation in the COG7 gene with a consistent phenotype including microcephaly, adducted thumbs, growth retardation, VSD and episodes of hyperthermia.

Eva Morava et al.

European journal of human genetics : EJHG, 15(6), 638-645 (2007-03-16)

We describe the clinical and biochemical characteristics in three patients from two different families diagnosed with Congenital Disorder of Glycosylation type IIe owing to a defect in Conserved Oligomeric Golgi complex (COG)7; one of the eight subunits of the COG.

A suite of novel human spermatozoal RNAs.

G Charles Ostermeier et al.

Journal of andrology, 26(1), 70-74 (2004-12-22)

We recently described a complex population of spermatozoal coding RNAs that are delivered to the oocyte on fertilization. These are derived throughout spermatogenesis, representing a record of past events. Recently, evidence has been provided that micro-RNAs are present in testes

Comparative analyses of the Conserved Oligomeric Golgi (COG) complex in vertebrates.

Rita Quental et al.

BMC evolutionary biology, 10, 212-212 (2010-07-17)

The Conserved Oligomeric Golgi (COG) complex is an eight-subunit assembly that localizes peripherally to Golgi membranes and is involved in retrograde vesicular trafficking. COG subunits are organized in two heterotrimeric groups, Cog2, -3, -4 and Cog5, -6, -7, linked by

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