SAB4200434

Anti-VHL antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-HRCA1, Anti-RCA1, Anti-VHL1, Anti-Von Hippel-Lindau tumor supressor
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~30 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells overexpressing human VHL; indirect immunofluorescence: 5-10 μg/mL using HeLa cells; western blot: 1.5-3.0 μg/mL using lysates of HEK-293T cells overexpressing human VHL
• UniProt accession no.: P40337
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... VHL(7428)

Description

General description

Von Hippel-Lindau tumor suppressor (VHL) is encoded by the gene mapped to human chromosome 3p25. The encoded protein is mainly expressed in adult brain and kidney. It is specifically localized on the cell membrane.

Immunogen

synthetic peptide corresponding to a sequence near the C-terminus of human VHL, conjugated to KLH. The corresponding sequence is identical in human VHL isoform 2 and highly conserved (94% sequence identity) in rat VHL.

Application

Anti-VHL antibody produced in rabbit has been used in immunoblotting, immunoprecipitation and immunofluorescence.

Biochem/physiol Actions

VHL acts as part of an E3 ubiquitin ligase that targets hypoxia-inducible factor α (HIF α) for rapid proteasomal degradation under normoxic conditions. VHL has been also identified as a key regulator of microtubule dynamic stability.

Von Hippel-Lindau tumor suppressor (VHL) acts as a tumor suppressor in some cancers. It might also play a vital role in signal transduction or cell adhesion. Mutation in the gene is associated with the development of sporadic cerebellar hemangioblastoma tumorigenesis and von Hippel-Lindau (VHL) disease. Interaction of the protein with hypoxia-inducible factor-1 (HIF-1) induces mammalian oxygen sensing.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable