SAB4200432

Anti-RASD2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-RAS homolog enriched in striatum, Anti-RASD family, member 2, Anti-RHES, Anti-TEM-2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen ~34 kDa
• species reactivity: human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells overexpressing human RASD2; indirect immunofluorescence: 5-10 μg/mL using HEK-293T cells overexpressing RASD2; western blot: 0.5-1 μg/mL using lysates of HEK-293T cells overexpressing human RASD2
• UniProt accession no.: Q96D21
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RASD2(23551)

Description

General description

RASD2 (Ras homolog enriched in striatum) is a member of the family of Ras small G proteins. RASD2 is a 266 amino acid GTP binding protein, which is expressed in the striatum. The gene is located on human chromosome 22 (q12.3).

Immunogen

synthetic peptide corresponding to a sequence near the C-terminus of human RASD2, conjugated to KLH. The corresponding sequence is highly conserved (83% sequence identity) in rat and mouse RASD2.

Application

Anti-RASD2 antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunoprecipitation

Biochem/physiol Actions

RASD2 (Ras homolog enriched in striatum) prevents the activation of G_αs and promotes dopamine D2 receptor-mediated activation of G_αi/o. It mediates mammalian target of rapamycin (mTOR) signaling and L-DOPA(L-3,4-dihydroxyphenylalanine)-induced dyskinesia. It is suggested to be the principal determinant of striatal mTOR activation. RASD2 is associated with Huntington′s disease (HD). Rhes-mHtt interaction is implicated in neuropathology of HD in the striatum.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable