SAB4200431
Anti-BRSK2 (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
Synonym(s):
Anti-BR serine/threonine kinase 2, SAD-A, PEN11B, Anti-SAD1, Anti-STK29
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.44
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~75 kDa
species reactivity
mouse, human, rat
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
dot blot: 1-2 μg/mL using HEK-293T cell lysates overexpressing mouse BRSK2.
indirect immunofluorescence: 0.1-0.2 μg/mL using HEK-293T cells overexpressing mouse BRSK2.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... BRSK2(9024)
mouse ... Brsk2(75770)
rat ... Brsk2(293631)
General description
Brain selective kinase 2 (BRSK2) (SAD-A) protein is a member of the family Ser/Thr 5′ adenosine monophosphate-activated protein kinase (AMPK) related kinases. This protein is specifically located in the spinal cord and brain of embryonic and postnatal animals. The BRSK2 gene is located on the human chromosome at 11p15.5.
Specificity
Anti-BRSK2 (N-terminal) specifically recognizes mouse BRSK2.
Immunogen
synthetic peptide corresponding to a sequence near the N-terminus of human BRSK2, conjugated to KLH. The corresponding sequence is identical in rat and mouse BRSK2.
Application
Anti-BRSK2 (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Brain selective kinase 2 (BRSK2) protein is phosphorylated by liver kinase B1 (LKB1) at Thr-174 thereby increasing its kinase activity. This protein is also phosphorylated at Thr-260 by cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) which is another upstream kinase. Brain selective kinase1/2 (BRSK1/2) phosphorylates downstream effectors like the cell cycle checkpoint kinase Wee1 and microtubule-associated protein tau. Phosphorylation of Wee1 by BRSK1/2 is essential for the regulation of its activity in polarized neurons and is an important step for the differentiation of polarized neurons.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs uponprolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Imre Noth et al.
The Lancet. Respiratory medicine, 1(4), 309-317 (2014-01-17)
Idiopathic pulmonary fibrosis (IPF) is a devastating disease that probably involves several genetic loci. Several rare genetic variants and one common single nucleotide polymorphism (SNP) of MUC5B have been associated with the disease. Our aim was to identify additional common
Masashi Kishi et al.
Science (New York, N.Y.), 307(5711), 929-932 (2005-02-12)
Electrical activity in neurons is generally initiated in dendritic processes then propagated along axons to synapses, where it is passed to other neurons. Major structural features of neurons-their dendrites and axons-are thus related to their fundamental functions: the receipt and
Zekun Guo et al.
Biochemical and biophysical research communications, 347(4), 867-871 (2006-07-28)
Brain selective kinase 2 (BRSK2) has been identified as a member of AMPK related kinases. LKB1 can phosphorylate the Thr174 of BRSK2, increasing its activity >50-fold. In this study, we identified cAMP-dependent protein kinase A (PKA) as another upstream kinase
Anthony P Barnes et al.
Cell, 129(3), 549-563 (2007-05-08)
The polarization of axon and dendrites underlies the ability of neurons to integrate and transmit information in the brain. We show here that the serine/threonine kinase LKB1, previously implicated in the establishment of epithelial polarity and control of cell growth
Myriam Müller et al.
Journal of cell science, 123(Pt 2), 286-294 (2009-12-23)
Wee1 is well characterized as a cell-cycle checkpoint kinase that regulates the entry into mitosis in dividing cells. Here we identify a novel function of Wee1 in postmitotic neurons during the establishment of distinct axonal and dendritic compartments, which is
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