SAB4200421

Anti-Lamin A/C R453W antibody, Mouse monoclonal

clone 12A-2F5, purified from hybridoma cell culture

• Synonym(s): Anti-LMN1, Anti-LMNA, Anti-Pre lamin-A/C, Monoclonal Anti-Lamin A/C R453W antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 12A-2F5, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~47 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 0.2-0.5 μg/mL using human Lamin A/C partial recombinant protein containing the R453W substitution
• isotype: IgG1
• UniProt accession no.: P02545
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LMNA(4000)

Description

General description

Monoclonal Anti-Lamin A/C R453W (mouse IgG1 isotype) is derived from the hybridoma 12A-2F5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Lamin A and lamin C belong to the A-type lamins. A-type lamins are characterized by an α-helical rod domain, a nuclear localization sequence, and a carboxy-terminal CAAX box. Lamin A is a structural protein of the nuclear lamina. The LMNA gene is mapped on the human chromosome at 1q22.

Specificity

Monoclonal Anti-Lamin A/C R453W recognizes a human Lamin A/C recombinant protein containing the R453W substitution. This antibody does not recognize the Lamin A/C wild-type protein.

Immunogen

synthetic peptide containing the R453W substitution of human Lamin A/C

Application

Monoclonal Anti-Lamin A/C R453W antibody produced in mouse may be used in:

• immunoblotting[1]
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Mutations in Lamin A and C have been linked to a variety of rare human diseases including muscular dystrophy, lipodystrophy, cardiomyopathy, neuropathy and progeroid syndromes collectively termed laminopathies and to premature aging, Hutchinson-Gilford progeria syndrome. Most diseases arise from dominant, missense mutations.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable