Monoclonal Anti-ZFYVE28/LST2 (mouse IgG1 isotype) is derived from the hybridoma LST139 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide. Zinc finger FYVE domain-containing protein 28 (ZFYVE28) localizes to the early endosome membrane in the absence of monoubiquitination while it localizes to the cytosol when it is monoubiquitinated. The ZFYVE28 gene is located on the human chromosome at 4p16.3.
Specificity
Monoclonal Anti-ZFYVE28/LST2 recognizes human and bovine ZFYVE28/LST2.
Immunogen
peptide corresponding to the N-terminus of human ZFYVE28 /LST2, conjugated to KLH. The corresponding sequence is identical in bovine, and has high homology in dog, rat and mouse ZFYVE28 /LST2.
Application
Monoclonal Anti-ZFYVE28/LST2 antibody produced in mouse may be used in immunoblotting and immunocytochemistry.
Biochem/physiol Actions
Zinc finger FYVE domain-containing protein 28 (ZFYVE28) contains one FYVE-type zinc finger, which mediates the interaction with phosphatidylinositol 3-phosphate (PI3P). ZFYVE28 functions as a negative regulator of epidermal growth factor receptor (EGFR) by promoting its degradation in endosomes when not monoubiquitinated on Lys-87.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Genetic screens performed in worms identified major regulators of the epidermal growth factor receptor (EGFR) pathway, including the ubiquitin ligase Cbl/SLI-1. Here we focus on the less-characterized Lst2 protein and confirm suppression of MAPK signals. Unexpectedly, human Lst2, a monoubiquitinylated
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