SAB4200417

Anti-XPO4 antibody, Mouse monoclonal

clone XP-12, purified from hybridoma cell culture

• Synonym(s): Anti-Exportin 4, Anti-KIAA1721, Monoclonal Anti-Exp4, Monoclonal Anti-XPO4 antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: XP-12, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~130 kDa
• species reactivity: bovine, chicken, hamster, monkey, canine, human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: suitable; western blot: 1.0-2.0 μg/mL using extracts of HeLa cells
• isotype: IgG1
• UniProt accession no.: Q9C0E2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... XPO4(64328)

Description

General description

Monoclonal Anti-XPO4 (mouse IgG1 isotype) is derived from the hybridoma XP-12 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide. Exportin 4 (XPO4) belongs to the karyopherins protein family. It is conserved amongst higher eukaryotes but, lacks obvious orthologues in yeast.

Immunogen

a peptide corresponding to a sequence at the C-terminus of human XPO4.

Application

Monoclonal Anti-XPO4 antibody produced in mouse has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Exportin 4 (XPO4) facilitates the nuclear export of eIF-5A (eukaryotic translation initiation factor 5A). In addition, it also mediates transport Sox proteins, as well as regulates Smad signaling. These functions of XPO4 suggests it to be a candidate tumor suppressor gene. Indeed, its involvement in the progression of human hepatocellular carcinomas (HCC) has been demonstrated, implicating this protein as a potential target for treatment of HCC.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable