SAB4200411

Anti-non-phospho-TDP-43 antibody produced in rabbit

IgG fraction of antiserum

• Synonym(s): Anti-TAR DNA binding protein 43, Anti-TARDP, Anti-TARDP43
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~43 kDa
• species reactivity: human, mouse
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:200 using using mouse kidney; indirect immunofluorescence: 1:200-1:400 using using HeLa cells; western blot: 1:500-1:1,000
• UniProt accession no.: Q13148
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TARDBP(23435) ; mouse ... Tardbp(230908)

Description

General description

TAR DNA binding protein, TARDP (TDP-43) belongs to the family of heterogeneous nuclear ribonucleoproteins (hnRNPs) that bind single stranded RNA. TDP-43 is predominantly localized to the nucleus. This gene is mapped to human chromosome 1p36.22.

Specificity

Anti-non-phospho-TDP-43 specifically recognizes human and mouse TDP-43 non-phosphorylated on Ser⁴⁰⁹ and Ser⁴¹. Detection of the non-phosphorylated TDP-43 band by immunoblotting is specifically inhibited by the non-phosphorylated TDP-43 immunizing peptide, but not by the corresponding mono-phosphorylated peptides TDP-43 [pSer⁴⁰⁹] and TDP-43 [pSer⁴¹⁰].

Immunogen

synthetic peptide containing non-phosphorylated Ser⁴⁰⁹ and Ser⁴¹⁰ of human TDP-43, conjugated to KLH. The corresponding sequence is identical in mouse TDP-43.

Application

Anti-non-phospho-TDP-43 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Members of the heterogeneous nuclear ribonucleoprotein (hnRNP) family play multiple roles in the generation and processing of RNA, including transcription, splicing, transport and stability. TAR DNA binding protein, TARDP (TDP-43) has been implicated in the transcription regulation of human immunodeficiency viruses (HIV). TDP-43 has been identified as the major ubiquinated component of cytoplasmic inclusions in frontotemporal lobe degeneration subtype frontotemporal lobar degeneration with ubiquitin-immunoreactive (ub-ir) inclusions (FTLD-U) and amyotrophic lateral sclerosis (ALS).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable