SAB4200410

Anti-Rheb (C-terminal region) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-RHEB2, Anti-Ras homolog enriched in brain
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~20 kDa
• species reactivity: human, rat
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using HEK-293T cells overexpressing human Rheb.; western blot: 0.5-1 μg/mL using cell lysates of HEK-293T overexpressing human Rheb.
• UniProt accession no.: Q15382
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RHEB(6009) ; rat ... Rheb(26954)

Description

General description

Ras homolog enriched in brain (Rheb), a member of the family of Ras small G-proteins, is a cellular immediate early gene in the brain. It has a C-terminal CaaX box.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human Rheb, conjugated to KLH. The corresponding sequence is identical in rat Rheb and highly conserved (94% sequence identity) in mouse Rheb.

Application

Anti-Rheb (C-terminal region) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Ras homolog enriched in brain (Rheb) plays an important role in cell growth, proliferation, apoptosis, autophagy and neuronal axonal regeneration. The effects of Rheb are mediated via the mammalian target of rapamycin (mTOR) protein kinase. Rheb activity in mTor signaling is regulated by a dual mechanism. Rheb appears to activate mTOR complex 1 (mTORC1) via direct binding to mTOR. Rheb is required for mTORC1 signaling and myelination during brain development.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable