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SAB4200408

Sigma-Aldrich

Anti-Dystrobrevin-α antibody produced in rabbit

enhanced validation

IgG fraction of antiserum

Synonym(s):

AntiDTNA

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:10,000-1:20,000 using HEK-293T cells overexpressing human dystrobrevin-a variant 6
western blot: 1:5,000-1:10,000 using lysates of HEK-293T cells overexpressing human dystrobrevin-a variant 6

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DTNA(1837)

General description

Dystrobrevin-α (α-DB or DTNA) belongs to the dystrobrevin (DB) subfamily of the dystrophin-related-protein family.α-DB is a component of the dystrophin-glycoprotein complex (DGC) that is located at the muscle sarcolemma and forms a transmembrane link between the cytoskeleton and the basal lamina. The DB family includes two isoforms α- and β-DB encoded by the different genes. α-DB is also abundantly expressed in developing neurons and in the adult central nervous system (CNS). The DTNA gene is located on the human chromosome at 18q12.1.

Specificity

Anti-Dystrobrevin-α specifically recognizes human dystrobrevin-α.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human dystrobrevin-a (DTNA), conjugated to KLH

Application

Anti-Dystrobrevin-α antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Dystrobrevin- α (α -DB) is involved in the formation and stability of synapses and the clustering of nicotinic acetylcholine receptors. Mutations in the DTNA gene are associated with congenital heart defects. Dystrobrevin is markedly reduced or absent in the sarcolemma of patients with Duchenne muscular dystrophy (DMD).

Physical form

solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Prenatal diagnosis of de novo interstitial deletions involving 5q23. 1-q23. 3 and 18q12. 1-q12. 3 by array CGH using uncultured amniocytes in a pregnancy with fetal interrupted aortic arch and atrial septal defect
Chen C P, et al.
Gene, 531(2), 496-501 (2013)
Dystrobrevins in muscle and non-muscle tissues
Rees M L J, et al.
Neuromuscular Disorders, 17(2), 123-134 (2007)
Function and genetics of dystrophin and dystrophin-related proteins in muscle
Blake D J, et al.
Physiological Reviews (2002)
Dystrobrevin deficiency at the sarcolemma of patients with muscular dystrophy
Metzinger L, et al.
Human Molecular Genetics, 6(7), 1185-1191 (1997)
T Haenggi et al.
Cellular and molecular life sciences : CMLS, 63(14), 1614-1631 (2006-05-20)
The dystrophin glycoprotein complex (DGC) is a multimeric protein assembly associated with either the X-linked cytoskeletal protein dystrophin or its autosomal homologue utrophin. In striated muscle cells, the DGC links the extracellular matrix to the actin cytoskeleton and mediates three

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