SAB4200407

Anti-phospho-PRAS40 (pSer¹⁸³) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-AKT1S1
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~40 kDa
• species reactivity: mouse, human, rat
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 5-10 μg/mL using formalin-fixed, paraffin-embedded human breast carcinoma.; immunoprecipitation (IP): 5-10 μg using extracts of HEK-293T cells.; western blot: 2-4 μg/mL using NIH3T3 and PC12 cell extracts.
• UniProt accession no.: Q96B36
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer183)
• Gene Information: human ... AKT1S1(84335) ; mouse ... Akt1s1(67605) ; rat ... Akt1s1(292887)

Description

General description

Proline-rich AKT1 substrate 1 (AKT1S1) gene encodes for proline-rich Akt-substrate of 40 kDa (PRAS40) and is located on the human chromosome at 19q13.33.

Specificity

Anti-phospho-PRAS40 (pSer183) specifically recognizes human, mouse and rat phosphorylated PRAS40(pSer183).

Immunogen

synthetic phospho-peptide containing phosphorylated Ser¹⁸³ of human PRAS40, conjugated to KLH. The corresponding sequence is identical in mouse and rat PRAS40.

Application

Anti-phospho-PRAS40 (pSer183) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunohistochemistry

Biochem/physiol Actions

Proline-rich Akt-substrate of 40 kDa (PRAS40) is a raptor binding protein that inhibits the mammalian target of rapamycin complex 1 (mTORC1) kinase activity. PRAS40 contains a TOR signaling (TOS) motif that mediates its binding to mTORC1. PRAS40 is involved in the protein kinase B (PI3KAkt/PKB) survival pathway. PRAS40 is phosphorylated on multiple residues including Ser-183, Ser-221 and Thr-246 in response to insulin and various growth factors like platelet-derived growth factor (PDGF) and nerve growth factor (NGF), or nutrients like glucose and amino acids. Phosphorylation of PRAS40 by Akt and mTORC1 disrupts the binding between mTORC1 and PRAS40, relieves the inhibitory effect of PRAS40 on mTORC1 activity, and facilitates the binding of PRAS40 to 14-3-3, a cytosolic anchor protein. PRAS40 binds to 14-3-3 with the help of amino acids and insulin which is partially inhibited by rapamycin.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable