SAB4200395

Anti-AMSH-LP / STAMBPL1 antibody, Mouse monoclonal

clone AMSH-LP-4, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-ALM alpha, Monoclonal Anti-AMSH-FP, Monoclonal Anti-AMSH-LP / STAMBPL1 antibody produced in mouse, Monoclonal Anti-STAM binding protein-like 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: AMSH-LP-4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: mouse, rat, human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using HEK-293T cells over-expressing AMSH-LP /STAMBPL1.
• isotype: IgG1
• UniProt accession no.: Q96FJ0
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... STAMBPL1(57559) ; mouse ... Stambpl1(76630)

Description

General description

Monoclonal Anti-AMSH-LP/STAMBPL1 (mouse IgG1 isotype) is derived from the hybridoma AMSH-LP-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Associated molecule with the SH3 domain of STAM-like protease (AMSH-LP), also known as signal-transducing adaptor molecule binding protein-like 1 (STAMBPL1), is a close homolog of AMSH. AMSH and AMSH-LP belong to the Jab1/MPN domain metalloenzyme (JAMM) domain metalloprotease family of Zn²⁺-dependent deubiquitinating enzymes (DUBs). It comprises a nuclear localization signal (NLS), an Mpr/Pad1/N-terminal (MPN) domain and a JAMM motif. The gene AMSH-LP is expressed in several human tissues. It is mapped to the human chromosome location 10q23.31.

Specificity

Monoclonal Anti-AMSH-LP /STAMBPL1 recognizes human AMSH-LP /STAMBPL1.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human STAMBPL1, conjugated to KLH. The corresponding sequence is identical in mouse, rat monkey, bovine and canine STAMBPL1.

Application

Monoclonal Anti-AMSH-LP/STAMBPL1 antibody produced in mouse may be used in immunoblotting.

Biochem/physiol Actions

Associated molecule with the SH3 domain of STAM-like protease (AMSH-LP) is involved in the deubiquitination of endosomal proteins and specifically cleaves Lys 63-linked polyubiquitin chains. It interacts with clathrin heavy chain and this interaction is essential for its endosomal localization. AMSH-LP positively regulates tumor growth factor β (TGF-β) signaling through interaction with inhibitory small mothers against decapentaplegic (I-SMAD). It functions as an adaptor for gene transcription and regulation system.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable