SAB4200392
Anti-BRSK1 antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody
Synonym(s):
Anti-BR serine-threonine kinase 1, Anti-SAD-B, Anti-SAD1 kinase
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.44
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~90 kDa
species reactivity
mouse
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.5 mg/mL
technique(s)
immunoprecipitation (IP): 10-20 μg using lysates of HEK-293T cells overexpressing mouse BRSK1
indirect immunofluorescence: 0.2-0.4 μg/mL using HEK-293T cells overexpressing mouse BRSK1
western blot: 2-4 μg/mL using extracts of mouse forebrain (S1 fraction)
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... BRSK1(84446)
mouse ... Brsk1(381979)
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General description
BRSK1 (BR serine/threonine kinase 1), also known as serine/threonine-protein kinase (SAD-1, SAD-B) and BRSK2 (SAD-A) belong to the family of Ser/Thr AMP-activated protein kinase (AMPK)-related kinases that are specifically expressed in the mammalian brain. BRSK1 gene is mapped to human chromosome 19q13.42. BRSK1 protein localizes in the synaptic vesicle in the hippocampus and cerebellum.
Specificity
Anti-BRSK1 specifically recognizes mouse BRSK1.
Immunogen
synthetic peptide corresponding to an internal sequence of human BRSK1, conjugated to KLH. The corresponding sequence is identical in mouse BRSK1.
Application
Anti-BRSK1 antibody is suitable for use in western blot (2-4 μg/mL using S1 fraction extracts of mouse forebrain). The product can also be used for immunoprecipitation (10-20 μg) and indirect immunofluorescence (0.2-0.4 μg/mL) using HEK-293T cells overexpressing mouse BRSK1.
Biochem/physiol Actions
BRSK1 (BR serine/threonine kinase 1) is essential for the polarization of cortical neurons. Knock-out mice that lack both BRSK1 and BRSK2 have defects in neuronal polarity and die prematurely after birth. Liver kinase B1(LKB1) phosphorylates and activates the serine/threonine-protein (SAD) kinases at a specific threonine residue within the T-loop activation segment of the kinase domain. BRSK1/2 in turn phosphorylates downstream effectors such as the microtubule-associated protein, tau and the cell cycle checkpoint kinase, Wee1-like protein kinase (Wee1). Phosphorylation of Wee1 by BRSK1/2 is required to regulate its activity and differentiation of polarized neurons. Also, BRSK1/SADB is localized to centrosomes and to control centrosome duplication. SADB phosphorylates γ-tubulin on Ser131 residue, suggesting that it controls centrosome homeostasis by regulating the phosphorylation of γ -tubulin.
BRSK1 is a serine/threonine kinase that facilitates the G(2)/M cell cycle arrest upon UV- or methyl methane sulfonate-induced DNA damage. This kinase also modulates the polarity of neurons and duplication of centrosomes. Anti-BRSK1 antibody is specific for mouse BRSK1. The antibody is also expected to react with human BRSK1. In immunoblotting, detection of the BRSK1 band is specifically inhibited by the BRSK1 immunizing peptide.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Genetics of reproductive lifespan.
Patricia Hartge
Nature genetics, 41(6), 637-638 (2009-05-28)
Arantza Rodríguez-Asiain et al.
Biochimica et biophysica acta, 1811(12), 1124-1135 (2011-10-25)
Brain specific kinases 1 and 2 (BRSK1/2, also named SAD kinases) are serine-threonine kinases specifically expressed in the brain, and activated by LKB1-mediated phosphorylation of a threonine residue at their T-loop (Thr189/174 in human BRSK1/2). BRSKs are crucial for establishing
María Alvarado-Kristensson et al.
Nature cell biology, 11(9), 1081-1092 (2009-08-04)
Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a
Rui Lu et al.
The Journal of biological chemistry, 279(30), 31164-31170 (2004-05-20)
Checkpoint activation by DNA damage during G(2) prevents activation of cyclin B/Cdc2 complexes, and as a consequence, mitotic entry is blocked. Although initiation and maintenance of G(2) arrest are known to be regulated by at least two distinct signaling pathways
Masashi Kishi et al.
Science (New York, N.Y.), 307(5711), 929-932 (2005-02-12)
Electrical activity in neurons is generally initiated in dendritic processes then propagated along axons to synapses, where it is passed to other neurons. Major structural features of neurons-their dendrites and axons-are thus related to their fundamental functions: the receipt and
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