SAB4200386
Anti-SLC1A1 (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
Synonym(s):
Anti-EAAC1, Anti-EAAT3, Anti-Solute carrier family 1, member 1
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~70 kDa
species reactivity
rat, human, mouse
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 2-4 μg/mL using Neuro-2a cells.
western blot: 1.5-3.0 μg/mL using postnatal (PN3) rat brain (S1 fraction) extracts, and 0.5-1.0 mg/mL using HEK-293T cell lysate over-expressing human SLC1A1.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SMTN(6525)
mouse ... Smtn(29856)
rat ... Smtn(289734)
General description
Solute carrier family 1 member 1 (SLC1A1) (also known as EAAC1, EAAT3) is localized at the postsynaptic site of neurons, whereas GLAST/EAAT1 and GLT1/EAAT2 are expressed at excitatory synapses by surrounding astrocytes. SLC1A1/EAAC1 is a glutamate transporter, expressed at moderate levels in the adult brain, mainly in the hippocampus, basal ganglia and olfactory bulb. The EAAT3 gene is mapped to human chromosome 9p24.2.
Specificity
Anti-SLC1A1(C-terminal) specifically recognizes human, rat and mouse SLC1A1.
Immunogen
synthetic peptide corresponding to a sequence at the C-terminal of human SLC1A1, conjugated to KLH. The corresponding sequence is identical in mouse and rat SLC1A1.
Application
Anti-SLC1A1 (C-terminal) antibody produced in rabbit has been used in:
- immunocytochemistry
- immunoprecipitation
- immunoblotting
- immunofluorescence
Biochem/physiol Actions
Solute carrier family 1 member 1 (SLC1A1) neural activity is highly regulated by intracellular signaling pathways including α protein kinase C (PKC α), phosphatidylinositol-3-kinase (PI3K), and glial factors, resulting in rapid changes in the trafficking of the transporter and its membrane location. It is considered to make a minor contribution to glutamate removal from the synapse and plays a key role in glutamate-mediated neuroplasticity. Hemi-deletion of the SLC1A1 gene may contribute to the pathophysiology of schizophrenia. The levels of EAAC1 are elevated in the plasma membrane when subjected to in vivo, long term potentiation or contextual fear conditioning. SLC1A1 displays increased expression in clear cell renal cell carcinoma (ccRCC).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Sercan Ergün et al.
Turkish journal of medical sciences, 49(2), 531-537 (2019-03-14)
This study aimed to comparatively analyze the expression levels of the SLC1A1 gene in renal specimens from tumors and adjacent healthy kidney tissues of patients with clear cell renal cell carcinoma (ccRCC). Nineteen patients diagnosed with ccRCC were included in
Parisa Afshari et al.
Molecular neuropsychiatry, 1(3), 125-144 (2015-09-19)
We have recently described a hemi-deletion on chromosome 9p24.2 at the SLC1A1 gene locus and its co-segregation with schizophrenia in an extended Palauan pedigree. This finding represents a point of convergence for several pathophysiological models of schizophrenia. The present report
Ivan Domith et al.
Journal of neurochemistry, 144(4), 408-420 (2017-11-23)
Vitamin C (in the reduced form ascorbate or in the oxidized form dehydroascorbate) is implicated in signaling events throughout the central nervous system (CNS). In the retina, a high-affinity transport system for ascorbate has been described and glutamatergic signaling has
Jonathan Levenson et al.
Nature neuroscience, 5(2), 155-161 (2002-01-15)
Induction and expression of long-term potentiation (LTP) in area CA1 of the hippocampus require the coordinated regulation of several cellular processes. We found that LTP in area CA1 was associated with an N-methyl-D-aspartate (NMDA) receptor-dependent increase in glutamate uptake. The
André Nieoullon et al.
Journal of neurochemistry, 98(4), 1007-1018 (2006-06-28)
EAAC1/EAAT3 is a transporter of glutamate (Glu) present at the post-synaptic neuronal element, in opposition to the two other main transporters, GLAST/EAAT1 and GLT1/EAAT2, expressed at the excitatory amino acid (EAA) synapse by surrounding astrocytes. Although, in the adult, EAAC1/EAAT3
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