SAB4200385

Monoclonal Anti-LMAN2L/VIPL antibody produced in mouse

clone VIPL-12, tissue culture supernatant

• Synonym(s): Monoclonal Anti-VIP36-like protein, Monoclonal Anti-lectin, Monoclonal Anti-mannose-binding 2-like
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: tissue culture supernatant
• antibody product type: primary antibodies
• clone: VIPL-12, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~34 kDa
• species reactivity: human, rat, mouse
• technique(s): western blot: 1:100-1:200 using HEK-293T cells over-expressing human LMAN2L/VIPL.
• isotype: IgM
• UniProt accession no.: Q9H0V9
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LMAN2L(81562) ; mouse ... Lman2l(214895) ; rat ... Lman2l(301343)

Description

General description

Lectin mannose binding 2-like (LMAN2L), also known as vesicular integral-membrane protein 36(VIP36) like protein (VIPL), is encoded by the gene mapped to human chromosome 2q11.2. It is a high-mannose type I membrane glycoprotein and is a member of the L-type lectin family. LMAN2L is expressed in wide variety of tissues.

Monoclonal Anti-LMAN2L/VIPL (mouse IgM isotype) is derived from the hybridoma VIPL-12 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide.

Specificity

Monoclonal Anti-LMAN2L/VIPL recognizes human LMAN2L/VIPL.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminal of human LMAN2L, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine and canine LMAN2L.

Application

Monoclonal Anti-LMAN2L/VIPL antibody produced in mouse may be used in immunoblotting.

Biochem/physiol Actions

Lectin mannose binding 2-like (LMAN2L) is a cargo receptor in the endoplasmic reticulum which has a role in glycoprotein transport. It may be implicated in targeting folded glycoproteins to the Golgi and misfolded glycoproteins to the ubiquitin-proteasome pathway. LMAN2L expression has been associated with bipolar disorder.

Lectin mannose-binding 2-like (LMAN2L) contains a luminal carbohydrate recognition domain (CRD) that binds to high-mannose type sugars in a Ca²⁺-dependent manner.

Physical form

Solution containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up toone month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation beforeuse. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable