SAB4200381

Anti-LAYN (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Layilin
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~43 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using whole extracts of human HepG2 or HEK-293T cells.
• UniProt accession no.: Q6UX15
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LAYN(143903)

Description

General description

Layilin (LAYN) is a cell surface hyaluronan receptor, initially recognized as a binding partner of talin found in membrane ruffles of spreading cells. This transmembrane protein is broadly expressed in different cell types and tissue extracts. The protein LAYN belongs to the hyaluronan (HA)-binding protein family. The gene LAYN is mapped to the human chromosome location 11q23.1.

Specificity

Anti-LAYN (C-terminal) recognizes human LAYN.

Immunogen

synthetic peptide corresponding to the C-terminal region of human LAYN, conjugated to KLH. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Application

Anti-LAYN (C-terminal) antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Layilin (LAYN) binds to hyaluronan, a ubiquitous extracellular matrix component involved in the invasion of a variety of tumor cells, through its extracellular domain. The protein is implicated in cell adhesion and motility by interacting with the cytoskeletal-membrane linker proteins including merlin and radixin, through its cytoplasmic domain. The LAYN gene is overexpressed in human lung carcinoma A549. LAYN down-regulation by RNA interference inhibits invasion and lymphatic metastasis of A549 cells. The protein LAYN transfers signals from the extracellular matrix (ECM) to the cell cytoskeleton.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage,freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discardedif not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable