SAB4200378

Anti-SMAD1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-BSP1, Anti-JV4-1, Anti-JV41, Anti-MADH1, Anti-MADR1, Anti-SMAD family member 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: mouse, human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 1-2 μg using lysates of HEK-293T cells over-expressing human SMAD1.; western blot (chemiluminescent): 0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human SMAD1.
• UniProt accession no.: Q15797
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SMAD1(4086) ; mouse ... 17125(Smad1)

Description

General description

Mothers against decapentaplegic homolog 1 (SMAD1) belongs to receptor-activated Smads (R-SMADs) subfamily. SMAD1 gene is mapped to human chromosome 4q31.21. The domain organization comprises of Mad homology 1 (MH1) domain andnuclear localisation signal (NLS) in the N-terminus. The C-terminal region has the Mad homology 1 (MH2), leucine-rich nuclear export signal (NES) and a proline-tyrosine (PY) motif.

Specificity

Anti-SMAD1 recognizes human SMAD1.

Immunogen

peptide corresponding to an internal region of human SMAD1, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in rat.

Application

Anti-SMAD1 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Mothers against decapentaplegic homolog 1 (SMAD1) is involved in mediating the signals of the bone morphogenetic proteins (BMP) and undergoes activation post phosphorylation by BMP receptor kinase. SMAD1 is a target for SMAD-specific E3 ubiquitin ligases, such as Smad ubiquitin regulatory factor 1 (SMURF1 and SMURF2) and undergoes ubiquitination and proteasome-mediated degradation. The phosphorylated form of SMAD1 forms a complex with SMAD4, which is important for its function in transcription regulation. SMAD1 is an oncogene which favors cancer cell growth and invasion especially in Glioma. It is a potential biomarker Diabetic nephropathy (DN pathology) along with urinary IgG4. An elevated extracellular signal-regulated kinases (ERKs) activity lead to higher activation of SMAD1 signaling which is implicated in the pathogenesis Cardio‐facio‐cutaneous (CFC) syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. Variants of SMAD1 and other SMAD genes are implicated in pulmonary arterial hypertension (PAH). SMADs in general mediate transmission of signals from the transforming growth factor-β (TGFβ) to the nucleus, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in workingaliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable