SAB4200377
Anti-SMAD1 (Internal region) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
Synonym(s):
Anti-BSP1, Anti-JV4-1, Anti-JV41, Anti-MADH1, Anti-MADR1, Anti-SMAD family member 1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~60 kDa
species reactivity
mouse, rat, human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 1-2 μg using lysates of HEK-293T cells over-expressing human SMAD1.
indirect immunofluorescence: 2.5-5.0 μg/mL using rat NRK cells.
western blot: 1-2 μg/mL using whole extracts of mouse F9 cells.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SMAD1(4086)
mouse ... Smad1(17125)
rat ... Smad1(25671)
General description
Mothers against decapentaplegic homolog 1 (SMAD1) belongs to receptor-activated Smads (R-SMADs) subfamily. It comprises Mad homology 1 (MH1) and nuclear localisation signal (NLS) in the N-terminus. The C-terminal region has the Mad homology 1 (MH2). SMAD1 also possesses leucine-rich nuclear export signal (NES) and a proline-tyrosine (PY) motif. SMAD1 gene is mapped to human chromosome 4q31.21.
Specificity
Anti-SMAD1 (internal region) recognizes human, mouse and rat SMAD1.
Immunogen
peptide corresponding to an internal region of human SMAD1, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in rat.
Application
Anti-SMAD1 (Internal region) antibody produced in rat may be used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
Biochem/physiol Actions
Mothers against decapentaplegic homolog (SMADs), in general, mediate transmission of signals from the transforming growth factor-β (TGFβ) to the nucleus, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD1 is involved in mediating the signals of the bone morphogenetic proteins (BMP) and undergoes activation post phosphorylation by BMP receptor kinase. The phosphorylated form of SMAD1 forms a complex with SMAD4, which is important for its function in transcription regulation. SMAD1 is a target for SMAD-specific E3 ubiquitin ligases, such as Smad ubiquitin regulatory factor 1 (SMURF1 and SMURF2) and undergoes ubiquitination and proteasome-mediated degradation. SMAD1 is an oncogene, which favors cancer cell growth and invasion, especially in glioma. It is a potential biomarker in diabetic nephropathy (DN) pathology along with urinary IgG4. An elevated extracellular signal-regulated kinases (ERKs) lead to enhanced activation SMAD1 signaling, which is observed in the pathogenesis of Cardio‐facio‐cutaneous (CFC) syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. Variants of SMAD1 and other SMAD genes are implicated in pulmonary arterial hypertension (PAH).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Assessment of the association between SMAD1 and HHIP gene variation and non-syndromic cleft-lip palate in Chilean case-parent trios
Suazo J, et al.
Genetics and Molecular Biology, 639-642 (2008)
Toshio Doi et al.
Diabetes, 67(5), 986-993 (2018-03-02)
Diabetic nephropathy (DN) is the major cause of end-stage kidney disease, but early biomarkers of DN risk are limited. Herein we examine urinary IgG4 and Smad1 as additional early DN biomarkers. We recruited 815 patients with type 2 diabetes; 554
Zhenguo Zeng et al.
Medicine, 99(10), e19451-e19451 (2020-03-10)
Non-small cell lung cancer (NSCLC) is the major cause of cancer mortality worldwide. Though multidisciplinary therapies have been widely used for NSCLC, its overall prognosis remains very poor, presumably owing to lack of effective prognostic biomarkers. SMAD, a well-known transcription
A Moustakas et al.
Journal of cell science, 114(Pt 24), 4359-4369 (2002-01-17)
Smad proteins transduce signals from transforming growth factor-beta (TGF-beta) superfamily ligands that regulate cell proliferation, differentiation and death through activation of receptor serine/threonine kinases. Phosphorylation of receptor-activated Smads (R-Smads) leads to formation of complexes with the common mediator Smad (Co-Smad)
Md Talat Nasim et al.
Human mutation, 32(12), 1385-1389 (2011-09-08)
Heterozygous germline mutations of BMPR2 contribute to familial clustering of pulmonary arterial hypertension (PAH). To further explore the genetic basis of PAH in isolated cases, we undertook a candidate gene analysis to identify potentially deleterious variation. Members of the bone
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