SAB4200375

Anti-UGGT1 (mouse) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-HUGT1, Anti-UDP-glucose glycoprotein glucosyltransferase 1, Anti-UGCGL1, Anti-UGT1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~170 kDa
• species reactivity: rat, mouse
• concentration: ~1.0 mg/mL
• technique(s): western blot: 2.5-5.0 μg/mL using whole extracts of mouse RAG or rat NRK cells.
• UniProt accession no.: Q6P5E4
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Uggt1(320011) ; rat ... Uggt1(171129)

Description

General description

Uridine diphosphate (UDP)-glucose:glycoprotein glucosyltransferase 1 (UGGT1) is a large soluble protein of the endoplasmic reticulum (ER). It contains a N-terminal folding sensor domain and a C-terminal catalytic domain that transfers glucose residues. It is a member of the glycosyltransferase family 24.

Specificity

Anti-UGGT1 (mouse) recognizes mouse and rat UGGT1.

Immunogen

synthetic peptide corresponding to the C-terminal region of mouse UGGT1, conjugated to KLH. The corresponding sequence differs by one amino acid in rat.

Application

Anti-UGGT1 (mouse) antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Uridine diphosphate (UDP)-glucose:glycoprotein glucosyltransferase 1 (UGGT1) that selectively re-glucosylates unfolded glycoproteins, thus providing quality control for protein transport out of the endoplasmic reticulum (ER). It re-glucosylates incompletely folded glycoproteins to monoglucosylated intermediates. Re-glucosylated proteins are recognized by ER-resident carbohydrate-binding molecular chaperons, calnexin or calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable