SAB4200372

Anti-WASH1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-FAM39E, Anti-WAS protein family homolog 1, Anti-WASH
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~72 kDa
• species reactivity: human, rat, mouse
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of rat NRK cells.; western blot: 2-4 μg/mL using whole extracts of HEK-293T cells over-expressing mouse WASH1.
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... WASH1(100287171) ; mouse ... ORF19(68767) ; rat ... 367328(Wash2)

Description

General description

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog) is a new member of the WASP family. Similar to other WASP family members, it contains a C-terminal for ′WH2, connecting and acidic′ (WCA) domain that binds to Actin related protein 2/actin related protein 3 (Arp2/3). In addition, WASH1 also contains a short proline-rich region, a unique N-terminal domain termed WASH-homology domain (WAHD1), and a tubulin-binding region.

Immunogen

synthetic peptide corresponding to an internal region of human WASH1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Application

Anti-WASH1 antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Biochem/physiol Actions

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), is a nucleation-promoting factor at the surface of endosomes. It recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of endosomes. WASH1 forms part of a multiprotein complex composed of FAM21, KIAA1033 strumpellin and WASH-interacting protein (SWIP) and coiled coil domain containing 53 (CCDC53). It associates with tubulin and localizes to early and recycling endosomes, where together with the Arp2/3 complex and actin, it is required for maintaining the shape of the endosomal compartment and the regulation of the retrograde transport.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable