SAB4200371

Anti-MAPKAP1 / SIN1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-MEKK2-interacting protein 1, Anti-MIP1, Anti-Mitogen-activated protein kinase associated protein 1, Anti-SAPK-Interacting protein 1, Anti-SIN1b, Anti-SIN1g
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~72 kDa
• species reactivity: human, mouse, rat
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of mouse C2C12 cells.; western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human MAPKAP1.
• UniProt accession no.: Q9BPZ7
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAPKAP1(79109) ; mouse ... Mapkap1(227743) ; rat ... Mapkap1(296648)

Description

General description

Mitogen-activated protein kinase associated protein 1 (MAPKAP1 )/stress-activated protein kinase-interacting protein 1 (SIN1) is an essential subunit of the mammalian target of rapamycin complex 2 (mTORC2) complex.

Immunogen

synthetic peptide corresponding to an internal region within human MAPKAP1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, canine and bovine.

Application

Anti-MAPKAP1/SIN1 antibody is suitable for use in immunoprecipitation (5-10 μg using lysates of mouse C2C12 cells) and western blot (0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human MAPKAP1).

Biochem/physiol Actions

Mitogen-activated protein kinase associated protein 1 (MAPKAP1 )/stress-activated protein kinase-interacting protein 1 (SIN1) regulates cell survival and cytoskeletal organization. It maintains mammalian target of rapamycin complex 2 (mTORC2) integrity and modulates Akt phosphorylation. Knockdown of SIN1 results in the disruption of the rictor-mTOR interaction and in defective Akt-Ser473 phosphorylation.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable