SAB4200364

Anti-PIWIL1 antibody, Mouse monoclonal

clone PIWIL1-6, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-PIWIL1 antibody produced in mouse, Anti-HIWI, Anti-MIWI, Anti-PIWI, Anti-piwi-like 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: PIWIL1-6, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~95 kDa
• species reactivity: mouse, human
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin^®-Peroxidase staining system; western blot: 2.5-5.0 μg/mL using whole extracts of mouse testis or HEK-293T cells over-expressing PIWIL1
• isotype: IgG1
• UniProt accession no.: Q96J94
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PIWIL1(9271) ; mouse ... Piwil1(57749)

Description

General description

Monoclonal Anti-PIWIL1 (mouse IgG1 isotype) is derived from the hybridoma PIWIL1-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to keyhole limpet hemocyanin (KLH). The PIWI-like protein 1 (PIWIL1) belongs to the Argonaute protein family. This gene is located on human chromosome 12q24.33. PIWIL1 protein has 861 amino acid residues and possess a molecular weight of 98.6?kDa. It is expressed in prostate, ovary, brain, liver, heart, kidney and skeletal muscle.

Immunogen

synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence is identical in mouse PIWIL1.

Application

Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in various immunochemical techniques including immunoblotting (~95 kDa) and immunohistochemistry.

Biochem/physiol Actions

Piwi proteins and piRNAs have been implicated in epigenetic control of gene expression, transposon silencing and translation regulation. Piwi proteins participates in germline development and gametogenesis of many metazoan species. PIWI-like protein 1 (PIWIL1) is also involved in stem cell proliferation, embryogenesis, growth and development, along with differentiation and maturation in numerous organisms. It can act as a molecular marker for forecasting the prognosis of colorectal cancer (CRC) patients.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable