SAB4200359

Anti-AMSH/STAMBP antibody, Mouse monoclonal

clone AMSH-10, purified from hybridoma cell culture

• Synonym(s): Anti-Associated Molecule with the SH3 domain of STAM, Anti-STAM binding protein, Anti-STAMBP
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: AMSH-10, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of human HEK-293T cells; western blot: 1-2 μg/mL using whole cell extracts of HEK-293T cells over-expressing AMSH/ STAMBP
• isotype: IgG1
• UniProt accession no.: O95630
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... STAMBP(10617)

Description

General description

Monoclonal Anti-AMSH/STAMBP (mouse IgG1 isotype) is derived from the hybridoma AMSH-10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Associated molecule with the SH3 domain of STAM (signal-transducing adaptor molecule) or STAM binding protein belongs to the JAMM domain metalloprotease family of Zn²⁺-dependent deubiquitinating enzymes (DUBs). AMSH is composed of multiple protein-protein interaction domains. It contains a nuclear localization signal (NLS), a microtubule-interacting and transport (MIT) domain, a clathrin-binding domain and a signal-transducing adaptor molecule (STAM) interacting motif. AMSH interacts directly with clathrin. Through its MIT domain, AMSH interacts with various charged multivesicular body proteins called chromatin-modifying proteins (CHMPs). CHMPs are constituents of the endosomal sorting complex required for transport (ESCRT) III complex. The AMSH gene is mapped to the human chromosome location 2p13.1.

Specificity

Monoclonal Anti-AMSH/ STAMBP recognizes human AMSH/ STAMBP.

Immunogen

synthetic peptide corresponding to the N-terminal region of human STAMBP, conjugated to KLH. The corresponding sequence is identical in canine and bovine, and differs by a single amino acid in mouse and by 2 amino acids in rat STAMBP.

Application

Monoclonal Anti-AMSH/STAMBP antibody produced in mouse may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Associated molecule with the SH3 domain of STAM (signal-transducing adaptor molecule (AMSH) is involved in the deubiquitination of the endosomal cargo before lysosomal degradation. The interaction of AMSH with the clathrin domain is essential for itsendosomal localization. AMSH specifically cleaves Lys-63-linked polyubiquitin chains and is implicated in the endocytic sorting of several receptors including G protein-coupled receptors and receptor tyrosine kinases. AMSH is also involved in the modulation of the calcium-sensing receptor, epidermal growth factor, the δ-opioid receptor and the protease-activated receptor 2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuoususe, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable