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Key Documents

SAB4200350

Sigma-Aldrich

Anti-ALS2 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

Synonym(s):

Anti-ALS2CR6, Anti-ALSJ, Anti-Alsin, Anti-IAHSP, Anti-PLSJ

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~185 kDa (An additional weak band of ~150 kDa may be observed in some cell extracts.)

species reactivity

mouse, rat, human

concentration

~1.5 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using NIH3T3 cells
western blot: 1.5-3.0 μg/mL using HEK-293T cell lysates over-expressing human ALS2 or rat cerebellum extracts (S1 fraction).

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ALS2(57679)
mouse ... Als2(74018)
rat ... Als2(363235)

Immunogen

synthetic peptide corresponding to a sequence near the C-terminal region of rat ALS2, conjugated to KLH. The corresponding sequence is identical in mouse and highly conserved (single amino acid substitution) in human ALS2.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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So Morishita et al.
FEBS letters, 593(2), 230-241 (2018-11-30)
Macropinocytosis is a nonspecific bulk uptake of extracellular fluid. During endosome maturation, the Rab5-to-Rab7 switch machinery executes the conversion from early to late endosomes. However, how the Rab switch works during macropinosome maturation remains unclear. Here, we elucidate the Rab

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