SAB4200342

Anti-SMAD3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-HSPC193, Anti-HsT17436, Anti-JV15-2, Anti-MADH3, SMAD family member 3, Anti-mothers against decapentaplegic homolog 3
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~55 kDa
• species reactivity: mouse, rat, human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human SMAD3.
• UniProt accession no.: P84022
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SMAD3(4088) ; mouse ... Smad3(17127) ; rat ... Smad3(25631)

Description

General description

SMAD family member 3 (SMAD3) belongs to the receptor-regulated SMADs (R-SMADs) family. It is encoded by the gene mapped to human chromosome 15q22.33. SMAD3 is expressed in various tissues and is characterized with two conserved MH1 domains at N-terminal end and a MH2 domain at C-terminal end.

Specificity

Anti-SMAD3 recognizes human SMAD3.

Immunogen

peptide corresponding to an internal region of human SMAD3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, dog, pig and bovine.

Biochem/physiol Actions

SMAD family member 3 (SMAD3) functions as an intracellular signaling molecule in the transforming growth factor β (TGF-β) pathway. Phosphorylated SMAD3 can form a complex with SMAD4, which translocates to the nucleus and regulates gene expression. SMAD3-SMAD4 complex can also interact with c-Jun and c-Fos at the activator protein 1 (AP-1) /SMAD site to regulate TGF-β-mediated transcription. SMAD3 has an inhibitory effect on wound healing. It also serves as a regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. SMAD3 gene polymorphisms leads to the development of osteoarthritis (OA).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable