SAB4200336

Anti-GUSB (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-β-D-Glucuronidase, Anti-β-Glucuronidase, Anti-β-g1, Anti-MPS7
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 78 kDa
• species reactivity: rat, human
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 10-20 μg/mL using biotin / ExtrAvidin^®-Peroxidase staining of heat-retrieved formalin-fixed, paraffin-embedded rat liver sections; western blot: 2-4 μg/mL using whole extracts of human HepG2 cells
• UniProt accession no.: P08236
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GUSB(2990) ; mouse ... Gusb(110006)

Description

General description

Glucuronidase β (GUSB), an exoglycosidase, is a member of the glycosyl hydrolase 2 family. The GUSB protein has a homotetrameric structure that is found in the lysosome. It contains three structural domains, such as a jelly roll barrel, an immunoglobulin constant domain and a triosephosphate isomerase (TIM) barrel respectively. The gene GUSB is mapped to the human chromosome location 7q11.21.

Specificity

Anti-GUSB (C-terminal) recognizes human and rat GUSB.

Immunogen

a synthetic peptide corresponding to a sequence at the C-terminal region of human GUSB, conjugated to KLH. The corresponding sequence is identical in mouse and differs by a single amino acid in rat.

Application

Anti-GUSB (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Glucuronidase β (GUSB) is a hydrolase that degrades glycosaminoglycans (GAGs), such as heparan sulfate, dermatan sulfate and chondroitin-4,6-sulfate. In the absence of GUS, only partial degradation of GAGs takes place. This leads to the accumulation of GAGs in the lysosomes of many tissues, leading to cellular and organ dysfunction. Mutations in this gene lead to mucopolysaccharidosis type VII (Sly syndrome). Several pseudogenes of this locus are present in the human genome.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable