SAB4200324

Anti-PABPN1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-OPMD, Anti-PAB2, Anti-PABP2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~55 kDa
• species reactivity: human, mouse
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed MCF7 cells; western blot: 2-4 μg/mL using MCF7 or RAW-264 cell lysates
• UniProt accession no.: Q86U42
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PABPN1(8106) ; mouse ... Pabpn1(54196) ; rat ... Pabpn1(116697)

Description

General description

Poly(A) binding protein nuclear (PABPN1) is a ubiquitously expressed nuclear protein that binds specifically and with a high affinity to poly(A) tails at the 3′ ends of mRNAs. PABPN1 contains a single RNP domain that separates a glutamate-rich N-terminal domain from an arginine rich C-terminal domain. PABPN1 is encoded by the gene mapped to human chromosome 14q11.2.

Immunogen

synthetic peptide corresponding to a sequence at the C- terminal region of human PABPN1, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Application

Anti-PABPN1 antibody produced in rabbit has been used in several immunochemical techniques including immunoblotting (~ 55 kDa) and immunofluorescence.

Biochem/physiol Actions

Poly(A) binding protein nuclear (PABPN1) plays a vital role in progressive and efficient polymerization of poly(A) tails on the 3′ ends of eukaryotic genes and controls the size of the poly(A) tail to about 250 nt. The ribonucleoprotein (RNP) domain and the C-terminal domain are required for binding to poly(A), and the N-terminus is essential for the stimulation of polyadenylation. Abnormal expansion of a (GCG)n trinucleotide repeat in the coding region of PABPN1 gene leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable